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* 2 tubes sorted into PBSE + 1% BSA (sat in tube at least 1 hour prior) # Full PI NeuN+ CTX from AD patient 18-99 (100,000) # Full PI NeuN- CTX from AD patient 18-99 (15,000) * Spun down at 900 RCF for 10 min at 4 C in bucket rotor * Resuspended in PBS + 1% BSA (100 ul for NeuN+, 30 ul for NeuN-), filtered through CellTrics 20 um filter, counted on BioRad TC20 ** NeuN+ was 350/ul ** NeuN- was 450/ul * Aliquoted NeuN+ in 5 ul volume, 15 tubes (Labeled +) * Aliquoted NeuN- in 2 ul volume, 10 tubes (Labeled -)
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