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==LiuKai1-4 Plates: NexteraXT Library Preparation== *Repeated Nextera on Normalized Samples prepared by Derek (note: First attempt at Nextera showed smears above 800bp, indicating bad stock of transposase - repeated with fresh stock) *Gel purified PCR products twice with 0.8x beads and resuspended in 10ul for each library: **LiuKai_1: Nu-iPSC early stage cells **LiuKai_2: Nu-iPSC late stage cellss **LiuKai_3: Normal iPSC early stage cells **LiuKai_4: Normal iPSC late stage cells Note: The subset of cDNA samples chosen for further processing was decided by Liu Kai Ran 1ul of each library on gel: [[File:12012014_LiuKai_Miseq.jpg]]
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