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Blue:RNA-Seq Libraries:05282013
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'''Library:''' BL_UHRR_IVT_0528 '''Purpose:''' Test the IVT RNA-seq protocol on 1ng and 10ng of UHRR using either a one-step or a two-step polyA T7 promoter adaptor addition. Also tested random cDNA synthesis using N6 and N9 primers. Results: Only two-step protocol worked. Both N6 and N9 primers appeared to work for fragment amplification between 200-600bp. Note: Used T20V.id for barcoding two-step protocol. T20V.ID1 = 10ng N6 Library T20V.ID2 = 1ng N6 Library T20V.ID4 = 10ng N9 Library T20V.ID5 = 1ng N9 Library [[File:05282013.pdf]]
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