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Blue:RNAseq Primer List
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==Adaptor Structure== [http://genome-tech.ucsd.edu/LabNotes/index.php/Rui:Toto-TSO_RNAseq_protocol Rui's totoRNAseq Page] ==totoRNA 5' primer sequence== * The 5' TSO adaptors list {| {{table}} border=1 | align="center" style="background:#f0f0f0;"|'''Tube ID''' | align="center" style="background:#f0f0f0;"|'''Primer full name''' | align="center" style="background:#f0f0f0;"|'''Primer full sequence''' | align="center" style="background:#f0f0f0;"|'''PE_F-N10''' | align="center" style="background:#f0f0f0;"|'''Barcode''' | align="center" style="background:#f0f0f0;"|'''TSO''' |- | TSO.r04||iso_B_TSO_N10||iso-dCiso-dGiso-dCACACTCTTTCCCTACACGACGNNNUNNNNUNNNrGrGrG||/5Me-isodC//iisodG//iMe-isodC/ACACTCTTTCCCTACACGACGNNN/ideoxyU/NNNN/ideoxyU/NNN||\||rGrGrG |- | TSO.r05||IsoL_B_TSO_N8||T-iSp18-ACACTCTTTCCCTACACGACGNNNNUNNNNrGrG+G||T/iSp18/ACACTCTTTCCCTACACGACGNNNN/ideoxyU/NNNNrGrG+G|||\||rGrG+G |- | TSO.r04_N6||iso_B_TSO_N6||isodC-isodG-isodC-ACACTCTTTCCCTACACGACGNNNUNNNrGrGrG||/5Me-isodC//iisodG//iMe-isodC/ACACTCTTTCCCTACACGACGNNN/ideoxyU/NNNrGrGrG|||\||rGrGrG |- | TSO.r06||iso_SMARTer II A||isodC-isodG-isodC-AAGCAGTGGTATCAACGCAGAGTACrGrGrG||/5Me-isodC//iisodG//iMe-isodC/AAGCAGTGGTATCAACGCAGAGTACrGrGrG|||\|||rGrGrG |- | |||||||||| |- | PB_PCR.F||PB_PCR.F||AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG||||\|| |- | PB_PCR.F*||PB_PCR.Fmod||AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG||A*A*T*GATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG||\|| |- | P1-STRT||P1-STRT||AATGATACGGCGACCACCGAGATCTAAGCAGTGGTATCAACGCAGAGT||||\|| |- | ILMN_PCR.F*||ILMN_PCR.Fmod||AATGATACGGCGACCACCGAGATC||A*A*T*GATACGGCGACCACCGAGATC||\|| |} ==totoRNA 3' primer sequence== * The 3' T20 adaptors list {| {{table}} border=1 | align="center" style="background:#f0f0f0;"|'''Tube ID''' | align="center" style="background:#f0f0f0;"|'''Primer full name''' | align="center" style="background:#f0f0f0;"|'''Primer full sequence''' | align="center" style="background:#f0f0f0;"|'''PE_R''' | align="center" style="background:#f0f0f0;"|'''Barcode''' | align="center" style="background:#f0f0f0;"|'''T20V''' |- | T20VN.r01||T20VN_PE_R||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTVN||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT||||TTTTTTTTTTTTTTTTTTTTVN |- | T20V.id01||T20V.BC8.PE_R.Indx1||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTCTGAGACTTTTTTTTTTTTTTTTTTTTV||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT||TCTGAGAC||TTTTTTTTTTTTTTTTTTTTV |- | T20V.id02||T20V.BC8.PE_R.Indx2||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGCCCAAGTTTTTTTTTTTTTTTTTTTTV||||TGCCCAAG|| |- | T20V.id03||T20V.BC8.PE_R.Indx3||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGACTCGCTTTTTTTTTTTTTTTTTTTTTV||||GACTCGCT|| |- | T20V.id04||T20V.BC8.PE_R.Indx4||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCACTCCATTTTTTTTTTTTTTTTTTTTV||||CCACTCCA|| |- | T20V.id05||T20V.BC8.PE_R.Indx5||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAACAGGGTTTTTTTTTTTTTTTTTTTTV||||GAACAGGG|| |- | T20V.id06||T20V.BC8.PE_R.Indx6||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTGCTGATTTTTTTTTTTTTTTTTTTTTV||||CTGCTGAT|| |- | T20V.id07||T20V.BC8.PE_R.Indx7||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTAGTATCTTTTTTTTTTTTTTTTTTTTV||||CTAGTATC|| |- | T20V.id08||T20V.BC8.PE_R.Indx8||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGCTGAAATTTTTTTTTTTTTTTTTTTTV||||GGCTGAAA|| |- | T20V.id09||T20V.BC8.PE_R.Indx9||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAGTGCTTTTTTTTTTTTTTTTTTTTTTV||||GAGTGCTT|| |- | T20V.id10||T20V.BC8.PE_R.Indx10||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATACGCCATTTTTTTTTTTTTTTTTTTTV||||ATACGCCA|| |- | T20V.id11||T20V.BC8.PE_R.Indx11||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATGCGTTATTTTTTTTTTTTTTTTTTTTV||||ATGCGTTA|| |- | T20V.id12||T20V.BC8.PE_R.Indx12||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTCACCCTTTTTTTTTTTTTTTTTTTTTTV||||TCACCCTT|| |- | T20V.id13||T20V.BC8.PE_R.Indx13||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCAACCGCTTTTTTTTTTTTTTTTTTTTV||||CCAACCGC|| |- | T20V.id14||T20V.BC8.PE_R.Indx14||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGATTACCTTTTTTTTTTTTTTTTTTTTV||||GGATTACC|| |- | T20V.id15||T20V.BC8.PE_R.Indx15||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCAGAGACATTTTTTTTTTTTTTTTTTTTV||||CAGAGACA|| |- | T20V.id16||T20V.BC8.PE_R.Indx16||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCAATAGTGTTTTTTTTTTTTTTTTTTTTV||||CAATAGTG|| |- | T20V.id17||T20V.BC8.PE_R.Indx17||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGTGGCCATTTTTTTTTTTTTTTTTTTTV||||GGTGGCCA|| |- | T20V.id18||T20V.BC8.PE_R.Indx18||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGAGGGAGTTTTTTTTTTTTTTTTTTTTV||||GGAGGGAG|| |- | T20V.id19||T20V.BC8.PE_R.Indx19||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTTGTCCTTTTTTTTTTTTTTTTTTTTTV||||GTTGTCCT|| |- | T20V.id20||T20V.BC8.PE_R.Indx20||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTTCGACCTTTTTTTTTTTTTTTTTTTTTV||||TTCGACC|| |- | T20V.id21||T20V.BC8.PE_R.Indx21||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCTTATTGTTTTTTTTTTTTTTTTTTTTV||||GCTTATTG|| |- | T20V.id22||T20V.BC8.PE_R.Indx22||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCAACCTTTTTTTTTTTTTTTTTTTTTTTV||||CAACCTTT|| |- | T20V.id23||T20V.BC8.PE_R.Indx23||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTTAGGTATTTTTTTTTTTTTTTTTTTTV||||CTTAGGTA|| |- | T20V.id24||T20V.BC8.PE_R.Indx24||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCTCACCATTTTTTTTTTTTTTTTTTTTV||||GCTCACCA|| |- | T20V.id25||T20V.BC8.PE_R.Indx25||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGAAATGCTTTTTTTTTTTTTTTTTTTTV||||AGAAATGC|| |- | T20V.id26||T20V.BC8.PE_R.Indx26||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTTACTGATTTTTTTTTTTTTTTTTTTTV||||TACTGATT|| |- | T20V.id27||T20V.BC8.PE_R.Indx27||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTCACGGGTTTTTTTTTTTTTTTTTTTTV||||CTCACGGG|| |- | T20V.id28||T20V.BC8.PE_R.Indx28||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCACTGTCATTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id29||T20V.BC8.PE_R.Indx29||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTTCTTGGGTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id30||T20V.BC8.PE_R.Indx30||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTACCGGCTTTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id31||T20V.BC8.PE_R.Indx31||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTAACCACATTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id32||T20V.BC8.PE_R.Indx32||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTACGCGCGTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id33||T20V.BC8.PE_R.Indx33||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCTCGATGTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id34||T20V.BC8.PE_R.Indx34||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGAGCTCATTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id35||T20V.BC8.PE_R.Indx35||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTATGGTCCTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id36||T20V.BC8.PE_R.Indx36||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGTCCGGCTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id37||T20V.BC8.PE_R.Indx37||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAAGGAATCTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id38||T20V.BC8.PE_R.Indx38||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCATCGTAGTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id39||T20V.BC8.PE_R.Indx39||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTGTTGAGTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id40||T20V.BC8.PE_R.Indx40||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATGTATCCTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id41||T20V.BC8.PE_R.Indx41||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGACAGGTTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id42||T20V.BC8.PE_R.Indx42||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTACGCCAATTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id43||T20V.BC8.PE_R.Indx43||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAAGGCAATTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id44||T20V.BC8.PE_R.Indx44||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCACCCGAGTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id45||T20V.BC8.PE_R.Indx45||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGTGTGTCTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id46||T20V.BC8.PE_R.Indx46||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTCTTGTGCTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id47||T20V.BC8.PE_R.Indx47||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGTACCTGTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id48||T20V.BC8.PE_R.Indx48||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGCGTCCTTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id49||T20V.BC8.PE_R.Indx49||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGAACTGTTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id50||T20V.BC8.PE_R.Indx50||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCAATGCTCTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id51||T20V.BC8.PE_R.Indx51||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTTAGGCGTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id52||T20V.BC8.PE_R.Indx52||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTAGACGCTTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id53||T20V.BC8.PE_R.Indx53||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTGGTGTGTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id54||T20V.BC8.PE_R.Indx54||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGCGGTAATTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id55||T20V.BC8.PE_R.Indx55||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCTTAAGTTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id56||T20V.BC8.PE_R.Indx56||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCGGTTTCTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id57||T20V.BC8.PE_R.Indx57||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAAGCCTAGTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id58||T20V.BC8.PE_R.Indx58||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTAGCCAGGTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id59||T20V.BC8.PE_R.Indx59||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTGGTAAATTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id60||T20V.BC8.PE_R.Indx60||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCACATAGGTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id61||T20V.BC8.PE_R.Indx61||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCCGGTGATTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id62||T20V.BC8.PE_R.Indx62||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCTAAACCTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id63||T20V.BC8.PE_R.Indx63||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCGTGTCCCTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id64||T20V.BC8.PE_R.Indx64||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGGGTAGGTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id65||T20V.BC8.PE_R.Indx65||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGGAGATGTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id66||T20V.BC8.PE_R.Indx66||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGGAAGTATTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id67||T20V.BC8.PE_R.Indx67||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGGCATGCTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id68||T20V.BC8.PE_R.Indx68||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTAGGTACTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id69||T20V.BC8.PE_R.Indx69||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCTGGATTTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id70||T20V.BC8.PE_R.Indx70||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGCTCCCTTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id71||T20V.BC8.PE_R.Indx71||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAAGCGCCCTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id72||T20V.BC8.PE_R.Indx72||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCGACGGATTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id73||T20V.BC8.PE_R.Indx73||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGATAGCGTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id74||T20V.BC8.PE_R.Indx74||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCCCAGTGTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id75||T20V.BC8.PE_R.Indx75||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTGAAGACTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id76||T20V.BC8.PE_R.Indx76||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAATCAGGATTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id77||T20V.BC8.PE_R.Indx77||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATGCTTCTTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id78||T20V.BC8.PE_R.Indx78||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCAAGCAGTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id79||T20V.BC8.PE_R.Indx79||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAAGTCGTTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id80||T20V.BC8.PE_R.Indx80||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTCGCCGCATTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id81||T20V.BC8.PE_R.Indx81||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTCAGTTACTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id82||T20V.BC8.PE_R.Indx82||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTAACGTGCTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id83||T20V.BC8.PE_R.Indx83||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGTAGGATTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id84||T20V.BC8.PE_R.Indx84||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGCACATCTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id85||T20V.BC8.PE_R.Indx85||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTGTCTTGTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id86||T20V.BC8.PE_R.Indx86||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGCAACCATTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id87||T20V.BC8.PE_R.Indx87||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCACTTGTCTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id88||T20V.BC8.PE_R.Indx88||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAGTTTGGTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id89||T20V.BC8.PE_R.Indx89||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTGCTGGATTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id90||T20V.BC8.PE_R.Indx90||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAGAGCGCTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id91||T20V.BC8.PE_R.Indx91||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCGAACATTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id92||T20V.BC8.PE_R.Indx92||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTACTATGCGTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id93||T20V.BC8.PE_R.Indx93||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTTAGCACTTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id94||T20V.BC8.PE_R.Indx94||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATGTCCATTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id95||T20V.BC8.PE_R.Indx95||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCCTGCCTTTTTTTTTTTTTTTTTTTTTV|||||| |- | T20V.id96||T20V.BC8.PE_R.Indx96||/5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTTGTCAGCTTTTTTTTTTTTTTTTTTTTV|||||| |- | |||||||||| |- | N2.id1||PCR_R.N2Ind1||CAAGCAGAAGACGGCATACGAGATCGTGATCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||CGTGAT||CTCGGCATTCCTGCTGAACCGCTCTT |- | N2.id2||PCR_R.N2Ind2||CAAGCAGAAGACGGCATACGAGATACATCGCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||ACATCG||CTCGGCATTCCTGCTGAACCGCTCTT |- | N2.id3||PCR_R.N2Ind3||CAAGCAGAAGACGGCATACGAGATGCCTAACTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||GCCTAA||CTCGGCATTCCTGCTGAACCGCTCTT |- | N2.id4||PCR_R.N2Ind4||CAAGCAGAAGACGGCATACGAGATTGGTCACTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||TGGTCA||CTCGGCATTCCTGCTGAACCGCTCTT |- | N2.id5||PCR_R.N2Ind5||CAAGCAGAAGACGGCATACGAGATCACTGTCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||CACTGT||CTCGGCATTCCTGCTGAACCGCTCTT |- | N2.id6||PCR_R.N2Ind6||CAAGCAGAAGACGGCATACGAGATATTGGCCTCGGCATTCCTGCTGAACCGCTCT||CAAGCAGAAGACGGCATACGAGAT||ATTGGC||CTCGGCATTCCTGCTGAACCGCTCTT |- | N2.id7||PCR_R.N2Ind7||CAAGCAGAAGACGGCATACGAGATGATCTGCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||GATCTG||CTCGGCATTCCTGCTGAACCGCTCTT |- | N2.id8||PCR_R.N2Ind8||CAAGCAGAAGACGGCATACGAGATTCAAGTCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||TCAAGT||CTCGGCATTCCTGCTGAACCGCTCTT |- | N2.id9||PCR_R.N2Ind9||CAAGCAGAAGACGGCATACGAGATCTGATCCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||CTGATC||CTCGGCATTCCTGCTGAACCGCTCTT |- | N2.id10||PCR_R.N2Ind10||CAAGCAGAAGACGGCATACGAGATAAGCTACTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||AAGCTA||CTCGGCATTCCTGCTGAACCGCTCTT |- | N2.id11||PCR_R.N2Ind11||CAAGCAGAAGACGGCATACGAGATGTAGCCCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||GTAGCC||CTCGGCATTCCTGCTGAACCGCTCTT |- | N2.id12||PCR_R.N2Ind12||CAAGCAGAAGACGGCATACGAGATTACAAGCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||TACAAG||CTCGGCATTCCTGCTGAACCGCTCTT |- | N2.id13||PCR_R.N2Ind13||||CAAGCAGAAGACGGCATACGAGAT||||CTCGGCATTCCTGCTGAACCGCTCTT |- | N2.id14||PCR_R.N2Ind14||||CAAGCAGAAGACGGCATACGAGAT||||CTCGGCATTCCTGCTGAACCGCTCTT |- | N2.id81||PCR_R.N2Ind81||CAAGCAGAAGACGGCATACGAGATCCAGGTCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||CCAGGT||CTCGGCATTCCTGCTGAACCGCTCTT |- | N2.id82||PCR_R.N2Ind82||CAAGCAGAAGACGGCATACGAGATCGTTTGCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||CGTTTG||CTCGGCATTCCTGCTGAACCGCTCTT |- | N2.id83||PCR_R.N2Ind83||CAAGCAGAAGACGGCATACGAGATGACCACCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||GACCAC||CTCGGCATTCCTGCTGAACCGCTCTT |- | N2.id84||PCR_R.N2Ind84||CAAGCAGAAGACGGCATACGAGATACAAGACTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||ACAAGA||CTCGGCATTCCTGCTGAACCGCTCTT |- | N2.id85||PCR_R.N2Ind85||CAAGCAGAAGACGGCATACGAGATACCGCACTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||ACCGCA||CTCGGCATTCCTGCTGAACCGCTCTT |- | N2.id86||PCR_R.N2Ind86||CAAGCAGAAGACGGCATACGAGATTGGGTACTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||TGGGTA||CTCGGCATTCCTGCTGAACCGCTCTT |- | N2.id87||PCR_R.N2Ind87||CAAGCAGAAGACGGCATACGAGATATTCCGCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||ATTCCG||CTCGGCATTCCTGCTGAACCGCTCTT |- | N2.id88||PCR_R.N2Ind88||CAAGCAGAAGACGGCATACGAGATGAATGTCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||GAATGT||CTCGGCATTCCTGCTGAACCGCTCTT |- | N2.id89||PCR_R.N2Ind89||CAAGCAGAAGACGGCATACGAGATGCTGATCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||GCTGAT||CTCGGCATTCCTGCTGAACCGCTCTT |- | N2.id90||PCR_R.N2Ind90||CAAGCAGAAGACGGCATACGAGATAGTGCTCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||AGTGCT||CTCGGCATTCCTGCTGAACCGCTCTT |- | N2.id91||PCR_R.N2Ind91||CAAGCAGAAGACGGCATACGAGATCAGGGACTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||CAGGGA||CTCGGCATTCCTGCTGAACCGCTCTT |- | N2.id92||PCR_R.N2Ind92||CAAGCAGAAGACGGCATACGAGATCATGCGCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||CATGCG||CTCGGCATTCCTGCTGAACCGCTCTT |- | N2.id93||PCR_R.N2Ind93||CAAGCAGAAGACGGCATACGAGATTGCCTACTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||TGCCTA||CTCGGCATTCCTGCTGAACCGCTCTT |- | N2.id94||PCR_R.N2Ind94||CAAGCAGAAGACGGCATACGAGATCTATACCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||CTATAC||CTCGGCATTCCTGCTGAACCGCTCTT |- | N2.id95||PCR_R.N2Ind95||CAAGCAGAAGACGGCATACGAGATCCGAGTCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||CCGAGT||CTCGGCATTCCTGCTGAACCGCTCTT |- | N2.id96||PCR_R.N2Ind96||CAAGCAGAAGACGGCATACGAGATACCTGCCTCGGCATTCCTGCTGAACCGCTCTT||CAAGCAGAAGACGGCATACGAGAT||ACCTGC||CTCGGCATTCCTGCTGAACCGCTCTT |- | |||||||||| |- | PGR_PCR_R||||CAAGCAGAAGACGGCATACGAGATCTCGGCATTCCTGCTGAACCGCTCTT||||CGAGAT|| |- | PGR_PCR_Rb||PGR_PCR_Rmod||CAAGCAGAAGACGGCATACGAGATCTCGGCATTCCTGCTGAACCGCTCTT||/5Phos/CAAGCAGAAGACGGCATACGAGATCTCGGCATTCCTGCTGAACCGCTCTT|||| |- | ILMN_PCR_Rb||ILMN_PCR_Rmod||CAAGCAGAAGACGGCATACGAGAT||/5Phos/CAAGCAGAAGACGGCATACGAGAT|||| |- |} ==IVT 5' primer sequence== * The 5' adaptors list {| {{table}} border=1 | align="center" style="background:#f0f0f0;"|'''Primer full name''' | align="center" style="background:#f0f0f0;"|'''Primer full sequence''' | align="center" style="background:#f0f0f0;"|'''PB_PCR_F Complementary Seq''' | align="center" style="background:#f0f0f0;"|'''Random Sequence''' |- | RL.IVT.N6||ACACTCTTTCCCTACACGACGNNNNNN||ACACTCTTTCCCTACACGACG||NNNNNN |- | RL.IVT.N9||ACACTCTTTCCCTACACGACGNNNNNNNNN||ACACTCTTTCCCTACACGACG||NNNNNNNNN |- |} ==IVT 3' primer sequence== * The 3' adaptors list {| {{table}} border=1 | align="center" style="background:#f0f0f0;"|'''Primer full name''' | align="center" style="background:#f0f0f0;"|'''Primer full sequence''' | align="center" style="background:#f0f0f0;"|'''T20 Complementary Seq''' | align="center" style="background:#f0f0f0;"|'''Novel T7 Seq''' |- | RL.Add.T7||CTTTAATTATGCTGAGTGATATCCCCGTAAGGACGACTTGGCGAGAA||GCATTCCTGCTGAACCGCTCTT||CTTTAATTATGCTGAGTGATATCCC |- |} == MARS-Seq/STRT-Seq/Smart-Seq2/Quartz-Seq Primer Sequences == {| {{table}} border=1 | align="center" style="background:#f0f0f0;"|'''Protocol''' | align="center" style="background:#f0f0f0;"|'''Primer full name''' | align="center" style="background:#f0f0f0;"|'''Primer full sequence''' | align="center" style="background:#f0f0f0;"|'''Modification''' | align="center" style="background:#f0f0f0;"|'''Barcode Seq''' |- | MARS-Seq||MARS_T20N_id01||CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTCGTGATNNNNTTTTTTTTTTTTTTTTTTTTN||N/A||CGTGAT |- | MARS-Seq||MARS_T20N_id02||CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTACATCGNNNNTTTTTTTTTTTTTTTTTTTTN||N/A||ACATCG |- | MARS-Seq||MARS_T20N_id03||CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTGCCTAANNNNTTTTTTTTTTTTTTTTTTTTN||N/A||GCCTAA |- | MARS-Seq||MARS_T20N_id04||CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTTGGTCANNNNTTTTTTTTTTTTTTTTTTTTN||N/A||TGGTCA |- | MARS-Seq||MARS_T20N_id05||CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTCACTGTNNNNTTTTTTTTTTTTTTTTTTTTN||N/A||CACTGT |- | MARS-Seq||MARS_T20N_id06||CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTATTGGCNNNNTTTTTTTTTTTTTTTTTTTTN||N/A||ATTGGC |- | MARS-Seq||MARS_T20N_id07||CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTGATCTGNNNNTTTTTTTTTTTTTTTTTTTTN||N/A||GATCTG |- | MARS-Seq||MARS_T20N_id08||CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTTCAAGTNNNNTTTTTTTTTTTTTTTTTTTTN||N/A||TCAAGT |- | MARS-Seq||MARS_T20N_id09||CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTCTGATCNNNNTTTTTTTTTTTTTTTTTTTTN||N/A||CTGATC |- | MARS-Seq||MARS_T20N_id10||CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTAAGCTANNNNTTTTTTTTTTTTTTTTTTTTN||N/A||AAGCTA |- | MARS-Seq||MARS_RT_2||TCTAGCCTTCTCGCAGCACATC||N/A|| |- | MARS-Seq||P5_Rd1_PCR_F||AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT||N/A|| |- | MARS-Seq||P7_Rd2_PCR_R||CAAGCAGAAGACGGCATACGAGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT||N/A|| |- | MARS-Seq||MARS_lig_NNNX4_ix1||/5Phos/GACTNNNAGATCGGAAGAGCGTCGTGTAG/3SpC3/||5Phos/3SpC3||GACT |- | MARS-Seq||MARS_lig_NNNX4_ix2||/5Phos/CATGNNNAGATCGGAAGAGCGTCGTGTAG/3SpC3/||5Phos/3SpC3||CATG |- | MARS-Seq||MARS_lig_NNNX4_ix3||/5Phos/CCAANNNAGATCGGAAGAGCGTCGTGTAG/3SpC3/||5Phos/3SpC3||CCAA |- | MARS-Seq||MARS_lig_NNNX4_ix4||/5Phos/CTGTNNNAGATCGGAAGAGCGTCGTGTAG/3SpC3/||5Phos/3SpC3||CTGT |- | MARS-Seq||MARS_lig_NNNX4_ix5||/5Phos/GTAGNNNAGATCGGAAGAGCGTCGTGTAG/3SpC3/||5Phos/3SpC3||GTAG |- | MARS-Seq||MARS_lig_NNNX4_ix6||/5Phos/TGATNNNAGATCGGAAGAGCGTCGTGTAG/3SpC3/||5Phos/3SpC3||TGAT |- | Smart-seq2||Smart2-T30VN||AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN||N/A|| |- | Smart-seq2||Smart2_ISPCR||AAGCAGTGGTATCAACGCAGAGT||N/A|| |- | Smart-Seq2||Smart2-TSO||AAGCAGTGGTATCAACGCAGAGTACATrGrG+G||rGrG+G (LNA)|| |- | UMI-STRT-Seq||C1-P1-RNA-TSO||Bio-AAUGAUACGGCGACCACCGAUNNNNNGGG||RNA|| |- | UMI-STRT-Seq||C1-P1-T31||Bio-AATGATACGGCGACCACCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT|||| |- | UMI-STRT-Seq||C1-P1-PCR-2||Bio-GAATGATACGGCGACCACCGAT|||| |- | UMI-STRT-Seq||C1-TN5-U||PHO-CTGTCTCTTATACACATCTGACGC|||| |- | Quartz-Seq||RT primer (WTA)||TATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTTTTTTTTTTTTTTTTTTTTTTT||N/A|| |- | Quartz-Seq||Tagging primer||TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTT||N/A|| |- | Quartz-Seq||Suppression primer||(NH2)-GTATAGAATTCGCGGCCGCTCGCGAT|||| |- |} ==NexteraXT Primers== {| {{table}} border=1 | align="center" style="background:#f0f0f0;"|'''Primer''' | align="center" style="background:#f0f0f0;"|'''Sequence''' | align="center" style="background:#f0f0f0;"|'''Index Sequence''' |- | NexteraXT.N7id01||CAAGCAGAAGACGGCATACGAGATCGTGATGTCTCGTGGGCTCGG||CGTGAT |- | NexteraXT.N7id02||CAAGCAGAAGACGGCATACGAGATACATCGGTCTCGTGGGCTCGG||ACATCG |- | NexteraXT.N7id03||CAAGCAGAAGACGGCATACGAGATCGACTCGTCTCGTGGGCTCGG||CGACTC |- | NexteraXT.N7id04||CAAGCAGAAGACGGCATACGAGATTGGTCAGTCTCGTGGGCTCGG||TGGTCA |- | NexteraXT.N7id05||CAAGCAGAAGACGGCATACGAGATCACTGTGTCTCGTGGGCTCGG||CACTGT |- | NexteraXT.N7id06||CAAGCAGAAGACGGCATACGAGATATTGGCGTCTCGTGGGCTCGG||ATTGGC |- | NexteraXT.N7id07||CAAGCAGAAGACGGCATACGAGATGATCTGGTCTCGTGGGCTCGG||GATCTG |- | NexteraXT.N7id08||CAAGCAGAAGACGGCATACGAGATTCAAGTGTCTCGTGGGCTCGG||TCAAGT |- | NexteraXT.N7id09||CAAGCAGAAGACGGCATACGAGATGAGCTCGTCTCGTGGGCTCGG||GAGCTC |- | NexteraXT.N7id10||CAAGCAGAAGACGGCATACGAGATAAGCTAGTCTCGTGGGCTCGG||AAGCTA |- | NexteraXT.N7id11||CAAGCAGAAGACGGCATACGAGATGTAGCCGTCTCGTGGGCTCGG||GTAGCC |- | NexteraXT.N7id12||CAAGCAGAAGACGGCATACGAGATTACAAGGTCTCGTGGGCTCGG||TACAAG |- | NexteraXT.N7id13||CAAGCAGAAGACGGCATACGAGATTCATGGGTCTCGTGGGCTCGG||TCATGG |- | NexteraXT.N7id14||CAAGCAGAAGACGGCATACGAGATTGTCTTGTCTCGTGGGCTCGG||TGTCTT |- | NexteraXT.N7id15||CAAGCAGAAGACGGCATACGAGATAGGAAGGTCTCGTGGGCTCGG||AGGAAG |- | NexteraXT.N7id16||CAAGCAGAAGACGGCATACGAGATAACCCCGTCTCGTGGGCTCGG||AACCCC |- | NexteraXT.N7id17||CAAGCAGAAGACGGCATACGAGATGATGAGGTCTCGTGGGCTCGG||GATGAG |- | NexteraXT.N7id18||CAAGCAGAAGACGGCATACGAGATTGAACTGTCTCGTGGGCTCGG||TGAACT |- | NexteraXT.N7id19||CAAGCAGAAGACGGCATACGAGATTGCGTCGTCTCGTGGGCTCGG||TGCGTC |- | NexteraXT.N7id20||CAAGCAGAAGACGGCATACGAGATGACAGGGTCTCGTGGGCTCGG||GACAGG |- | NexteraXT.N7id21||CAAGCAGAAGACGGCATACGAGATGGGTTGGTCTCGTGGGCTCGG||GGGTTG |- | NexteraXT.N7id22||CAAGCAGAAGACGGCATACGAGATTCCGAGGTCTCGTGGGCTCGG||TCCGAG |- | NexteraXT.N7id23||CAAGCAGAAGACGGCATACGAGATTTTCGAGTCTCGTGGGCTCGG||TTTCGA |- | NexteraXT.N7id24||CAAGCAGAAGACGGCATACGAGATGCGAATGTCTCGTGGGCTCGG||GCGAAT |- | NexteraXT.S501||AATGATACGGCGACCACCGAGATCTACACTAGATCGCTCGTCGGCAGCGTC||TAGATCGC |- |} ==Sequencing Primers== {| {{table}} border=1 | align="center" style="background:#f0f0f0;"|'''Primer full name''' | align="center" style="background:#f0f0f0;"|'''Primer full sequence''' | align="center" style="background:#f0f0f0;"|'''Protocol''' | align="center" style="background:#f0f0f0;"|'''Read''' |- | totoRNAseq Read 1 v2 || CCACCGAGATCTACACTCTTTCCCTACACGACG || totoRNAseq || Read1 |- | N2RevSeq2 || CTCGGCATTCCTGCTGAACCGCTCTTCCGATCT || totoRNAseq || Read2 |- | N2IndSeq || AAGAGCGGTTCAGCAGGAATGCCGAG || totoRNAseq || N2 index Read |- | STRT-SEQ v3 || GATCTAAGCAGTGGTATCAACGCAGAGTACGGG || totoRNAseq || Read1 |- |} ==Zhang Lab Indexes== [https://docs.google.com/spreadsheets/d/1F2L2NpqYCXYwYPk1WQdJDkj3yZKMJftYfeYUHtiXCL4/pubhtml# Index List]
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