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Daniel:Notebook/HiResChrPaint/2013-9-27
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=FISH= [[Daniel:Notebook/HiResChrPaint|Back to Calendar]] Using the slides from [[Daniel:Notebook/HiResChrPaint/2013-9-24#Fibroblast Adherence|Tuesday]], I'm going to do another FISH experiment. '''Samples''': *10 pmol [[Daniel:Notebook/HiResChrPaint/2013-7-17#Labeling Results|V6S3]], 10 pmol [[Daniel:Notebook/HiResChrPaint/2013-7-25#Labeling Results|V4S2]], 2.5 ug human Cot-1 DNA; *20 pmol [[Daniel:Notebook/HiResChrPaint/2013-9-5|BSPP-1-546]], 2.5 ug human Cot-1 DNA ==FISH Protocol== *Following modified [[Daniel:Protocols/FISH#Hybridization|FISH procedure]] ''Day 1'' 1. Allow stored slides to warm to room temperature 2. Incubate slides in 2X SSCT +50% (v/v) formamide for 2.5 minutes at 92 C; use pre-warmed solution 3. Incubate in 2X SSCT + 50% formamide at 60C for 20 minutes 4. Remove media from slides and allow to cool to room temperature 5. Add 25 uL hybridization cocktail to center of slide 6. Denature for 2.5 minutes at 92C; keep humid; used rock bath 7. Transfer slides to a humidified chamber and hybridize overnight at 42C Continued [[Daniel:Notebook/HiResChrPaint/2013-9-28|tomorrow]]
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