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=Dystrophin Probes= [[Daniel:Notebook/HiResChrPaint|Back to Calendar]] The dystrophin probes I designed [[Daniel:Notebook/HiResChrPaint/2014-7-10|last week]] arrived from IDT. Attached are their specs: *[[Media:IDT_PlateSpecs_IDTOrder.txt|IDT Order Specs]] The oligos arrived as dry oligos in a plate. The calculations I made are in the following text file: *[[Media:IDT_PlateSpecs_07172014.txt|IDT Plate Spec Calculations]] ==Oligo Pool Synthesis== #Resuspend each oligo to make them all 1 mM (See Plate Specs for values) #Combine 2 uL from each oligo ##Makes 12.5 ug/uL total pool ##Total volume will be 64 uL #Add 742.4 uL nfH2O, dilutes to 1 ug/uL total pool ##Create dilution series and measure in nanodrop ===Nanodrop Results=== {| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> |- style="font-size:12pt;font-weight:bold" align="center" valign="bottom" | width="78" height="15" | Dilution (X:1) | width="65" | ng/uL | width="65" | proj. ug/uL |- style="font-size:12pt" align="center" valign="bottom" | align="center" height="15" | 5 | align="center" | 79.6 | align="center" | 0.40 |- style="font-size:12pt" align="center" valign="bottom" | align="center" height="15" | 10 | align="center" | 38.2 | align="center" | 0.38 |- style="font-size:12pt" align="center" valign="bottom" | align="center" height="15" | 20 | align="center" | 19.3 | align="center" | 0.39 |- style="font-size:12pt" align="center" valign="bottom" | align="center" height="15" | 50 | align="center" | 7.4 | align="center" | 0.37 |- style="font-size:12pt" align="center" valign="bottom" | align="center" height="15" | 100 | align="center" | 3.7 | align="center" | 0.37 |- style="font-size:12pt" align="center" valign="bottom" |style="font-weight:bold" height="15" | Average | align="center" | |style="font-weight:bold" align="center" | 0.38 |} So I guess my calculations were a bit off, but 0.4 ug/uL is pretty good for FISH, especially considering I need 30 pmol per experiment (0.4 ug/uL ~60 pmol/uL). [[Category:HRCP]] [[Category:20140718]]
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