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=Trial Run ([[Daniel:Notebook/RNAFISH/2014-8-29|Started 08/29/2014]])= [[Daniel:Notebook/RNAFISH|Back to Calendar]] ==Post-Hybidization== #Remove rubber cement together with the chamber using a pair of fine forceps #Wash slides in 2X SSC at 37Β°C, 3 Γ 15 min (preferably with shaking) to remove hybridization mixture #Perform stringency washes in 0.1X SSC at 60Β°C, 2 Γ 5 min to remove nonspecific hybrids #Equilibrate in 2X SSC for 2 min #Add DAPI, let sit for at least 30 minutes #Seal the coverslip with nail polish; let sit 30 minutes ##Sections become fragile after the FISH; therefore, strong pressure on the coverslip should be avoided ##e.g., pressing the coverslip with the microscope objective when focusing often completely destroys the sample ==Microscopy== This sample was viewed over several days, they are listed here: <dynamicpagelist> category=RNAFISH category=Microscopy category=20140829 </dynamicpagelist> [[Category:RNAFISH]] [[Category:Hybridization]] [[Category:20140829]]
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