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Daniel:Protocols/ssDNA Column
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=ssDNA Column Purification (Zymo)= [[Daniel Jacobsen|Back to Main]] '''Zymo: D7010 (20); D7011 (50)''' ==Protocol== <ol> <li>Add 2 volumes '''Binding Buffer''' to the sample; mix well</li> <li>Transfer to '''IIC Column''' and centrifuge at 14000 rpm for 1 minute; '''SAVE THE FLOW THROUGH'''</li> <li>Add 1 volume 100% EtOH to flow through; mix well</li> <li>Transfer to '''IC Column''' and centrifuge at 14000 rpm for 1 minute; discard flow through</li> <li>Add 400 uL '''Prep Buffer''' and centrifuge at 14000 rpm for 1 minute; discard flow through</li> <li>Add 700 uL '''Wash Buffer''' and centrifuge at 14000 rpm for 1 minute; discard flow through</li> <li>Add 400 uL '''Wash Buffer''' and centrifuge at 14000 rpm for 1 minute; discard flow through</li> <li>Centrifuge empty column at 14000 rpm for 2 minutes</li> <li>Transfer to empty 1.5mL centrifuge tube (low bind)</li> <li>Add β₯ 6 uL nfH2O and centrifuge at 14000 rpm for 1 minute</li> </ol>
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