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EricChu:LabNotesMDA/2013-9-17
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== MDA Device v7 (PGP1 #15) with Tagmentation == * The following samples were labeled as EC-PGP1-Dev7-index#,index#-Sep17 and given to Alan for sequencing. * They could be pooled into the same lane as they all have different index #s. 5 million reads were asked. ==Size Selection== {| {{table}} |[[File:Sept17TBEgel1.jpg|400px]] || |[[File:Sept17TBEgel2.jpg|400px]] |- |[[File:Sept17TBEgel1cut.jpg|400px]] || |[[File:Sept17TBEgel2cut.jpg|400px]] |} == MDA Device v9 (PGP1 #17) with Tagmentation == * Repeating the experiment of 9-15-2013 * Device preparation was described in EricChu:LabNotesMDA/2013-5-20 * MDA recipe was followed to the protocol in EricChu:LabNotesMDA/2013-5-14 * Compare experiments of (1) v7 device with Tagmentation and (2) v9 device with Tagmentation * One PGP1 cell was used in this experiment. * Note 1: Valve above the mixing chamber was used. The valve was pumping every 5 seconds for one minute. * Note 2: After air pushed the ALS+cell, about 5 chambers were estimated empty (7 chambers were partially empty). They were then filled with ALS. * Note 3: Valve between ALS and NS was opened for 2 minutes before MDA mix was pushed into the MDA chambers.
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