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==qPCR Alu and Mito of MDA generated libraries on 1-30-2014== * These libraries were prepared by tagmentation. * 1ul of each library was used in a 20ul qPCR reaction. * 1ul 10ng human gDNA was used as control. [[File:02052014qPCR.jpg|600px]] * Alu primers were labeled black and Mito primers were labeled red. * Alu curves of both libraries on 1-30-2014 (labeled Hi and Lo) were amplified later than their corresponding Mito curves. However, as seen in gDNA, Alu should be amplified earlier than Mito. * In this experiment, most of the DNA were coming from mitochondrial DNA rather than chromosomal DNA.
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