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*[[Hosuk:Lab_Notes|LabNote]] ===Rolonies with fresh cells (96well plate)=== ====Goal==== *Produce rolonies with (relatively) fresh cells *Try invitrogen a-dUTP (from Dan) and compare to the current (brown vial) anaspect's one *Check if there are many ACTB from these fresh cell samples. ====Process==== *Got PGP1F from Alan, the cells were thaw two days ago *Split cells (10/16) on 8 wells in 96 well plate, 2 Mattek dishes **40uL per each well (+100uL media), 1mL per each Mattek dish *Fix cells (10/17) and proceed RT (9:30 pm)
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