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*[[Hosuk:Lab_Notes|LabNote]] ===Fixing cells in 96 well plate=== *I've split cells in 16 wells. *First 8 wells are fixed with 2 step, others with 1 step fixing. *For 1 stpe fixing ** Take out 150uL media, so there is 150uL in well. ** Add 100uL 10% formalin, so it is 4% formalin in well. *For 2 step fixing ** Take out 100uL media, so there is 200#uL in well. ** Add 20uL 37% formalin, so it is 1:10 ratio, as following the first rolony protocol that I've got. *4 wells in 1st, 2nd row --> 2 step fixing *4 wells in 3rd, 4th row --> 1 step fixing *4 wells in 1st, 3rd row --> Anaspec's a-dUTP *4 wells in 2nd, 4th row --> Invitrogen's a-dUTP
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