Editing
Hosuk:LabNotes/2013-11-12
Jump to navigation
Jump to search
Warning:
You are not logged in. Your IP address will be publicly visible if you make any edits. If you
log in
or
create an account
, your edits will be attributed to your username, along with other benefits.
Anti-spam check. Do
not
fill this in!
*[[Hosuk:Lab_Notes|LabNote]] ===Experimental Plan=== ====RT with 8 different RT probes==== *for RT from a specific location *Will be started 11/12 evening ~ finished at 11/14 (Th) *Pick the good wells (8 wells or 16 wells for ACTB RT probes, 4 wells for hexamer RT) out of 32 wells ====Padlock probe concentration==== *2uM , 0.66uM was working *1nM --> 12x10^10 numbers *MALAT1 was roughly 3700 numbers in one field of view (1mm^2) --> ~ 654,900 in one MatTek dishes *--> if there are 100 targets in Rolony --> 6.5 x 10^6 targets *0.1nM, 1nM, 10nM, 100nM, 1uM , 5uM for 1 padlock probes --> MALAT1 padlock probe *Use 1 MatTek Dish --> pick one of dishes from one made @11/11 (has been used so far) *will be done in this week ====Ampligase test==== *Just Do 2nd RCA and Ampligase, then image --> 2 MatTek Dishes --> #1 and #2@10/22 (has been used so far) **Try 2nd RCA, and anneal 2nd RCA detection probes **Use 3 padlock probes **Parameters **Temperature : 55C **Time : 4hr **Enzyme concentration : 5U (1uL of Ampligase) *Image 1st rolony --> Image padlock probes --> Image 2nd Rolony *will be done in next week *Optimize --> later
Summary:
Please note that all contributions to ZhangLabWiki may be edited, altered, or removed by other contributors. If you do not want your writing to be edited mercilessly, then do not submit it here.
You are also promising us that you wrote this yourself, or copied it from a public domain or similar free resource (see
ZhangLabWiki:Copyrights
for details).
Do not submit copyrighted work without permission!
Cancel
Editing help
(opens in new window)
Navigation menu
Personal tools
Not logged in
Talk
Contributions
Create account
Log in
Namespaces
Page
Discussion
English
Views
Read
Edit
View history
More
Search
Navigation
Main Page
Current events
Recent changes
Random page
Investigators
Matt Cai
Song Chen
Eric Chu
Dinh Diep
Elizabeth Duong
Shicheng Guo
Alan Fung
Daniel Jacobsen
Blue Lake
Huy Lam
Alice Li
Andrew Richards
Brandon Sos
Chris Wei
Yan Wu
Kun Zhang
Tools
What links here
Related changes
Special pages
Page information