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*[[Hosuk:Lab_Notes|LabNote]] ====Day 3==== *Jay finished last step, washing RCA and binding Cy3 probe, and image *Rolonies are pretty well generated inside cells, *and the signal is very strong, so there were almost no background *Even though there were some of debris, they could be come from fixing step. *No RCA signal were in -RT sample =====20x, +RT vs. -RT (1 wash)===== {| {{table}} |Raw images : [[Media:+RT_1wash_ch00.tif|+RT]], [[Media:-RT_1wash_ch00.tif|-RT]] |- |[[File:20x_1wash.png|500px]] |} =====20x, +RT, NA=0.4 vs. 0.8 (2 wash)===== {| {{table}} |Raw images : [[Media:20x_0.4NA_2wash_same setting_ch00.tif|NA=0.4]], [[Media:20x_0.8NA_2wash_same setting_ch00.tif|NA=0.8]] |- |[[File:20x_2wash_0.4NA_0.8NA.png|500px]] |} =====40x, +RT, NA=0.8, Cy3 vs. GFP channel (2 wash)===== {| {{table}} |Raw images : [[Media:40x_0.8NA_2wash_ch00.tif|Cy3]], [[Media:40x_0.8NA_2wash_GFPcontrol_ch00.tif|GFP]] |- |[[File:40x_2wash_0.8NA_Cy3_GFP.png|500px]] |} =====63x, +RT, NA=1.2, Cy3 (2 wash)===== {| {{table}} |Raw images : [[Media:63x_2wash_1.2NA_Cy3.tif|63x]] |- |[[File:63x_1.2NA_2wash_50msec_100EMgain_ch00.png|250px]] |}
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