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*[[Hosuk:Lab_Notes|LabNote]] ===42C RT incubation time and New CircLigase II Reaction Buffer Test=== *Test new, home made CircLigase II buffer in fixed cell sample *And 42C RT reaction temperature test *New reaction buffer is used for all samples. *#Dish1 : PGP1F, random hexamer, 37C RT temp. *#Dish2 : PGP1F, RAB7A specific RT primer, 37C RT temp. *#Dish3 : PGP1F, random hexamer, 42C RT temp. *#Dish4 : PGP1F, RAB7A specific RT primer, 42C RT temp. ====Result==== *RT at 42C incubation works well *New CircLigase II Buffer works well *Position specific RT primer of RAB7A (220bp) makes many rolonies. *And there are a few of RAB7A target detected, Iβm currently analyzing how many RAB7A targets are there. *Images with 63x have more Rolonies/cell, I think the reason is 63x has smaller field of view, so there are much fewer cells in a field of view, but 63x obj. can detect many rolony signals. *RAB7A targets are about ~5% out of 1st rolonies, which is always found in other experiments. [[File:RolonyCount_20140326_with3D.png|600px]]
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