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*[[Hosuk:Lab_Notes|LabNote]] ===Cancer Cell : Mutation detection test - 2nd Try=== *NCIH-1975 in 24 well plate *Fix : 04/08 *Finish all process 04/10 *Well 1, 2 : EGFR, BRAF RT Primers *Well 3, 4 : EGFR, BRAF RT Primers + BRAF Stop primer 200bp *Well 5, 6 : EGFR, BRAF RT Primers + BRAF Stop primer 200bp *Well 7, 8, 9, 10 : random hexamer [[File:24Well_2014-04-10.png|300px]] [[File:NCIH1975_2014-04-10_1stRolony_Count.png|150px]] ====Result for 1st Rolonies==== *Well 1 ~ Well 6 have similar number of rolonies, *which the numbers are much less than Well 7, 8, and 9. **(Well 10 has few, itβs because of mistake at RNaseH step) ===PGP1F sample for 13k Padlock probe test=== *Fix : 04/06, 2 dishes *Finish all process 04/10, imaging *S1 : incubate RCA primer at RT for 10min after incubating RCA primer at 60C oven for 15min. *S2 : Wash and add RCA mix right after incubating RCA primer at 60C oven for 15min. ====Result for 1st Rolonies==== *Both S1 (incubate RCA primer at RT for 10min), S2 looked similar amount of Rolonies *Both sample have pretty good 1st Rolonies! *I will use S2 for Ampligase and RCA with13k Padlock probes. [[File:PGP1F_2014-04-10_1stRolony_Count.png|400px]]
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