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*[[Hosuk:Lab_Notes|LabNote]] ===Deconvolution=== *Try deconvolution with the sample(07/04; S1 : T48 RT primer, S2 : rHexamer), especially 63x Zoom=4 images ====Procedure==== *Plug-in of ImageJ --> PSF by 'PSF Generator’ : ‘PSF Gibson and Lanni_Zoom4_Z00.tif’ **Set parameter for 63x, zoom=4, **2048x2048, z=21, and use Z00 *Plug-in of ImageJ --> Deconvolution by ‘DeconvolutionJ’ ====Result==== *Both images after deconvolution increase the number of rolony counted. *Counts **S1(T48 RT Primer) : 769 --> 907 (after deconvolution), 17.9% increase **S2(random hexamer) : 1462 --> 1933 (after deconvolution), 32.3% increase [[File:Deconv_S1_Pos3.jpg|1100px]] [[File:Deconv_S2_Pos2.jpg|1100px]]
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