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*[[Hosuk:Lab_Notes|LabNote]] ===1st Rolonies per Nuclues=== ====BA22==== =====Count Nucleus===== *Sample : HB22_S4_2014-12-12 (0.01% Pepsin for 5 min) (1st rolony count sample : Pos1) *Olympus Epi Image size : **1300 x 1030 px --> 1444 um x 1144 um --> 1.6416mm^2 **Nucleus count by BW and regionprop (result : 2015-01-09 folder, size>30) *** # of Nucleus = 1528 --> 930.799922 / mm^2 (--> 0.924 / 100um^2) =====Count 1st Rolony===== *Data : MIP_HBTissue_2014-12-12_S4_TX100_15m_Pepsin_0.01_5m_20x_4k_Pos1.tif *Run PISA --> # = 10261 *Confocal 20x obj. image field of view = 581.25 um x 581.25 um --> 0.337852 mm^2 **'''Estimated the number of nucleus in the 20x obj. image field of view = 930.8 x 0.338 = 314.47''' **'''Estimated the number of 1st Rolonies per nucleus = 10261/ 314.47 = 32.63''' ====BA8==== =====Count Nucleus===== *S1_RCA2014-12-19_2ndRCA2015-01-08_DAPI_Olympus_40x_Pos1.tif --> 43 *S1_RCA2014-12-19_2ndRCA2015-01-08_DAPI_Olympus_40x_Pos2.tif --> 47 *Olympus, 40x --> 1344 x 1024 pixel = 266.67 um x 203.17 um --> 0.0541793 mm^2 ** # of Nucleus = (average of two data) 45 --> 830.5748 / mm^2 =====Count 1st Rolony===== *Data : MIP_HBTissue_2014-12-19_S1_TX100_15m_0.01Pepsin_5min_20x_4k_Pos3.tif *Run PISA --> # = 11654 **'''Estimated the number of nucleus in the 20x obj. image field of view = 830.5748 x 0.338 = 280.61''' **'''Estimated the number of 1st Rolonies per nucleus = 11654 / 280.61 = 41.53'''
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