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*[[Hosuk:Lab_Notes|LabNote]] ===Imaging with Structural Illumination microscopy=== *New microscope in Yun’s lab : Zeiss, Apotome2 structural illumination microscopy *Image with 3 samples **PGP1F_S1_1st Rolony_2015-01-18 **BA8_S1_1st Rolony_2015-02-27 **BA8_S1_V4_2015-02-14 *SIL imaging condition **20x obj., Image size : 2752 x 2208 pixel, 0.23 um /pixel ** --> 632.96 x 507.84 um *Brain tissue section has a strong auto-fluoresence!, so we need image with 488 channel as a auto fluorescent signal. **Auto-fluorescent signal image(488 channel) was subtract from BA8, 2015-02-14 Image, but not from BA8, 2015-02-27 Image (we didn’t have enough time to re-image). So BA8, 2015-02-27 image potentially have auto-fluorescent image! ====Images==== =====Sample:PGP1F_S1_2015-01-18===== [[File:Imging_2015-03-26_PGP1F_S1_2015-01-18_Zeiss_SIL_20x.png|700px]] [[File:Imging_2015-03-26_PGP1F_S1_2015-01-18_Zeiss_SIL_20x_Detail1.png|800px]] =====Sample:BA8_1stRolony_S1_2015-02-27===== [[File:Imging_2015-03-26_BA8_1stRolony_S1_2015-02-27_Zeiss_SIL_20x.png|700px]] [[File:Imging_2015-03-26_BA8_1stRolony_S1_2015-02-27_Zeiss_SIL_20x_Detail1.png|800px]] =====Sample:BA8_CA12kNov_V4_S1_2015-02-14===== [[File:Imging_2015-03-26_BA8_CA12kNov_V4_S1_2015-02-14_Zeiss_SIL_20x.png|700px]] [[File:Imging_2015-03-26_BA8_CA12kNov_V4_S1_2015-02-14_Zeiss_SIL_20x_Detail1.png|800px]] ====Comparison==== *Imaging time (20x obj, 10 stacks, 3 colors) **Confocal 2048 x 2048 px : ~ 40 sec **Confocal 4096 x 4096 px : ~ 2min 40 sec **SIL 2752 x 2208 px : ~ 30 sec *Area of a field of view (20x obj, 10 stacks, 3 colors) **Confocal 2048 x 2048 px : 581 um x 581 um **Confocal 4096 x 4096 px : 581 um x 581 um **SIL 2752 x 2208 px : 632.96 x 507.84 um *Resolution quality : **Confocal 2048 x 2048 < SIL 20x < Confocal 4096 x 4096 [[File:Comparison_Leica_Confocal_20x_4k_2k_Zeiss_SIL_20x_2.png|1200px]]
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