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*[[Hosuk:Lab_Notes_MDA|LabNote]] ===MDA with PGP1F, 8th, Dev v9.1=== *Use new device ([[Hosuk:LabNotesMDA/2013-7-16|v9.1]]) ====Experiment==== =====KOH concentration for (ALS+Cell) mix===== *In 20uL reaction, the final concentration of KOH in ALS while denaturing is '''240mM'''. *In the v7.3 version, '''269mM''' of KOH in ALS buffer has used ([[Hosuk:LabNotesMDA/2013-7-2|v7.3 ring mixer device]]). *For this v9.1 device, '''286mM''' of KOH in ALS buffer is used (Cell : (ALS-Cell) = 1 : 5.247). *[[File:ALS_Recipe.png|600px]] *Start : 8:30pm 07/17, End : 9:00am 07/18 --> about 13hr reaction *qPCR run for verification ====Result==== *Amount of amplicons were good. (output channel 22 was clogged.) *Some of sample could be lost because of back flow as soon as opening valve *So the left 4 chamber were not filled well. [[File:Backflow_after_opening_valve_2013-07-18.png|400px]] *I didn't run MDA in ALS chamber, but the reaction will be done in ALS chamber as well for the next experiment. *Also I'll try to push the (ALS+Cell) solution into compartment with '''H2O''' to prevent sample loss like this. *[[File:qPCR_Result_MDAv9.1_2013-07-18_fig1.png|650px]] *[[File:qPCR_Result_MDAv9.1_2013-07-18_fig2_Table.png|400px]] *[[File:qPCR_Result_MDAv9.1_2013-07-18_fig3_History.png|600px]]
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