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20080716 primer to produce spacer:refer to LabNotes on [http://genome-tech.ucsd.edu/LabNotes/index.php/Jie:LabNotes/ExonomeSeq/2008-7-16] CirclehelperV4_short(20080825): AACAGTGCTCTTCCAGTCTACTAGCCTCATGCGTATCCGATC 20080904 Prmer used to amplify the cpg30k and cosmic3760. The amplicon will have 5-terminal phosphate, thus can be ligated after circlehelper insertion. AmpFV6.2_2: 5Phos/C*T*GCAGATGTTATCGAGGTC AmpRV6.2_2: 5Phos/CTGAATAGGAACGATGAGCC 20080918 confirmation primers for cpg30k. Primers targeting bisulfite conversed DNA to confirm the capture results of cpg30K. [[Media: 20080918_conirmed primers for cpg30k.xls]] 20080929 sapcer with length of 60mer that can be ligated without extension. Spacer_60mer_reverse: gacctcgataacatctgcagTGCAAAGTAGAACACCAGAACCAGTACAATCACCGTTGCGTTTGGTGAGAATCCTTCCAGCTGAATAGGAACGATGAGCC Spacer_60mer: 5β-Phos/ctggaaggattctcaccaaacgcaacggtgattgtactggttctggtgttctactttgca [[Media:20080929_spacer of 60mer.doc]] 20081019 confirmation primers for cpg30k from cpg28k library. [[Media: 20081019_conirmed primers for cpg30k from cpg28k.xls]] 20081019 positive control used for cosmic probe circlehelper insertion. cosmic_probe_control: GTAGACTGGAAGAGCACTGTTGTTGGAGGCTCATCGTTCCTATTCAGCTGCAGATGTTATCGAGGTCCGACGATCGGATACGCATGAGGCTA 20081024 more confirmation primers for cpg30k from cpg28k library. [[Media: 20081024_confirmed primers plate_2 for cpg30k from cpg28k.xls]] [[Media: 20081024_conirmed primers plate_3 for cpg30k from cpg28k.xls]]
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