Editing
Jie:LabNotes/sample record/sample received
Jump to navigation
Jump to search
Warning:
You are not logged in. Your IP address will be publicly visible if you make any edits. If you
log in
or
create an account
, your edits will be attributed to your username, along with other benefits.
Anti-spam check. Do
not
fill this in!
2008-07-24: From James Thomson, Biotechnology Center, Madison, WI. Two tubes tube1:IMR90 tube2:IMR90-04 2008-08-01: From James Thomson,Biotechnology Center, Madison, WI. Two tubes. Tube 1: IMR90P24, 7/29/08 tube 2: Qwell of le well plate, ipsIMR90CH, P28,18,4, 7/22/08 20080826: From Paul Ventura, Covaris, Inc. refer to LabNotes on [http://genome-tech.ucsd.edu/LabNotes/index.php/Jie:LabNotes/CpgSeq/2008-8-12] IMB90_iPS_sheared sequencing DNA. Nanodrop:dsDNA 19.8ng/ul; 260/280 1.07; 260/230 0.51 Hybrid_1_sheared sequencing DNA. Nanodrop:dsDNA 21.6ng/ul; 260/280 1.13; 260/230 0.56 20090515:From Yuan Gao E0H 45ng/ul 50ul; E4H 45ng/ul 50ul; E24H 50ng/ul 42ul; 20090518 from Kun DF iPS from Thomson lab refer to LabNotes on [http://genome-tech.ucsd.edu/LabNotes/index.php/Jie:LabNotes/CpgSeq/2009-5-29] foreskin 1.0 583ng/ul 1.93 1.16 DF6-9-9 P16(4) 0.3 504.8ng/ul 1.88 2.04 DF4-5-7 P16(4) 0.4 541ng/ul 1.86 1.99 DF6-9-12 p16(4) 0.4 425.3ng/ul 1.88 2.09 DF19-9-11 p17(4) 0.4 484.7ng/ul 1.89 2.04 DF19-9-7 p17(4) 0.3 460.3ng/ul 1.89 2.04 BJ 60ng/ul x 200ul BJ-iPS11 140ng/ul x 30ul BJ-iPS12 40ng/ul x 80ul HUES6 250ng/ul x 200ul NA21687*1 062507 241.1ng/ul 1.89 1.51 refer to LabNotes on [http://genome-tech.ucsd.edu/LabNotes/index.php/Jie:LabNotes/CpgSeq/2009-6-19] NA21781*1 102307 240ng/ul 1.89 1.26 NA21833*1 101807 253.9ng/ul 1.9 1.49 NA21660*1 053007 243.2ng/ul 1.92 1.66 NA21731*1 101807 217.4ng/ul 1.88 1.49 NA21846*1 100407 186.5ng/ul 1.89 1.47 NA21070*1 100406 329.7ng/ul 1.99 1.77 cell: hiPS 11 P17 hiPs 12 P20 2009-06-17: From Zhuohua Zhang. refer to LabNotes on [http://genome-tech.ucsd.edu/LabNotes/index.php/Jie:LabNotes/CpgSeq/2009-7-1] No 5028-1818 are unaffacted control tissues; No 1947-4526 are Parkinson's disease and Parkinson's disease/Lewy body disease. 5028: 320ng/ul; 4735: 260ng/ul; 4789: 280ng/ul; 5171: 420ng/ul; 1818: 480ng/ul; 1947: 360ng/ul; 1741: 560ng/ul; 4977: 260ng/ul; 4879: 340ng/ul; 4526: 380ng/ul. [[Image:20090617_gDNA from Zhuohua Zhang.jpg|200px]]20090617_gDNA from Zhuohua Zhang 2009-07-28: From kim Smoga-Otto of Thomson lab.refer to LabNotes on [http://genome-tech.ucsd.edu/LabNotes/index.php/Jie:LabNotes/CpgSeq/2009-8-10] two tubes of iPS-DF6.9h, passage 28. (17 Jul 09). 5 million cell/tube. 2009-07-28: From Jehyuk Lee from Harvard Medical school. PGP1_iPS1 gDNA: 47ng/ul x 50ul; PGP1_iPS1 mRNA: 476ng/ul x 20ul; PGP1_iPS1 ssDNA from mRNA: 146ng/ul x 10ul. 2009-08-04: From Kun (breast cancer cells from patient) refer to LabNotes on [http://genome-tech.ucsd.edu/LabNotes/index.php/Jie:LabNotes/CpgSeq/2009-8-7] 05192A18 05192B09 2009-09-04: From LD 353-03 53.7ng/ul LD351-01 73.6ng/ul MEC001 47.8ng/ul KaG001 31.9ng/ul 2009-10: from Harvard {| border="1" cellpadding="5" cellspacing="0" align="center" |- | align="center" style="background:#f0f0f0;"|''' ''' | align="center" style="background:#f0f0f0;"|'''sample ''' |align="center" style="background:#f0f0f0;"|'''sample concentration''' | align="center" style="background:#f0f0f0;"|'''260/280, 260/230''' | align="center" style="background:#f0f0f0;"|'''volume ''' |- |Harvard_1||B0047.1 ||927.1ng/ul||1.8/2.25||20ul |- |Harvard_2||B0060.1||206ng/ul ||1.92/1.56||20ul |- |Harvard_3||B0070.1||760ng/ul||1.9/2.1||20ul |- |Harvard_4||B0107.1||360ng/ul ||1.9/1.9||5ul |- |Harvard_5||B0108.1 ||266ng/ul ||1.9/1.5||15ul |- |Harvard_6||B0134.1||54.2ng/ul||1.9/1.2||40ul |- |Harvard_7||M0013.1 ||380ng/ul ||1.9/2.0||20ul |- |Harvard_8||M0077.3||47ng/ul ||1.9/1.2||40ul |- |Harvard_9||M0124.3 ||46ng/ul||1.9/1.1||40ul |- |Harvard_10||M0188.3||82ng/ul||1.9/1.6||30ul |- |} seq lib construction refer to LabNotes on [http://genome-tech.ucsd.edu/LabNotes/index.php/Jie:LabNotes/CpgSeq/2010-1-21] and [http://genome-tech.ucsd.edu/LabNotes/index.php/Jie:LabNotes/CpgSeq/2010-1-28] the sample sequencing tracking can be seen [[Media:20100210_harvard_sample_sequencing_tracking.xls]] 20090918 from cancer center The sample tracking can be seen [[Media:20090918_breast_cancer_samples.xls]] 20100121 from Y. Geno Shi in Harvard. refer to LabNotes on [http://genome-tech.ucsd.edu/LabNotes/index.php/Jie:LabNotes/CpgSeq/2010-1-22] ct1 mES gDNA 11.20.09: 120ng/ul Tet1-KD mES gDNA 11.20.09: 120ng/ul
Summary:
Please note that all contributions to ZhangLabWiki may be edited, altered, or removed by other contributors. If you do not want your writing to be edited mercilessly, then do not submit it here.
You are also promising us that you wrote this yourself, or copied it from a public domain or similar free resource (see
ZhangLabWiki:Copyrights
for details).
Do not submit copyrighted work without permission!
Cancel
Editing help
(opens in new window)
Navigation menu
Personal tools
Not logged in
Talk
Contributions
Create account
Log in
Namespaces
Page
Discussion
English
Views
Read
Edit
View history
More
Search
Navigation
Main Page
Current events
Recent changes
Random page
Investigators
Matt Cai
Song Chen
Eric Chu
Dinh Diep
Elizabeth Duong
Shicheng Guo
Alan Fung
Daniel Jacobsen
Blue Lake
Huy Lam
Alice Li
Andrew Richards
Brandon Sos
Chris Wei
Yan Wu
Kun Zhang
Tools
What links here
Related changes
Special pages
Page information