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==Exp. 1 Perform CES27k eSNP capture on PGP samples== Genomic targets: 800ng/3pg per haploid genome = 2.67x10^5 copies = 2.22x10~-19 moles Probes: 2.22x10~-19 moles * 22,000 * 125 = 1.22x10^-12 moles. {| border="1" cellpadding="5" cellspacing="0" align="center" |- | align="center" style="background:#f0f0f0;"|''' ''' | align="center" style="background:#f0f0f0;"|''' ''' | align="center" style="background:#f0f0f0;"|'''Template''' | align="center" style="background:#f0f0f0;"|'''10x buffer''' | align="center" style="background:#f0f0f0;"|'''CES27k probes (209nM, 01/28/08)''' | align="center" style="background:#f0f0f0;"|'''H2O''' |- |1||NA20431 gDNA(347ng/ul)||2.3ul||1ul||4.5ul||2.2ul |- |2||GM20431 cDNA (122ng/ul)||1.6ul||1ul||4.5ul||2.9ul |- |3||Hues36 gDNA(246ng/ul)||3.5ul||1ul||4.5ul||1.0ul |- |4||Hues36 dscDNA(80ng/ul, 08/22/07)||2.5ul||1ul||5ul||2.0ul |} 94C 5min->60C 40h-> add 1.0ul SLN mix (1U/ul stoffel, 0.5U/ul AmpLigase, 1uM dNTP) -> 65C 1h -> 37C 1min -> add 1.0ul of exo mix (Exo I, Exo III)->37c 2 hour -> 95C 5min -> 4C hold.
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