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{| {{table}} | Dec-12-2007||[[Kun:LabNotes/ExonomeSeq/2007-12-12|Probe prep for E55kv1 set]]|||||||| |- | Dec-14-2007||[[Kun:LabNotes/ExonomeSeq/2007-12-14|PAGE purification of probes]]|||||||| |- | Dec-16-2007||[[Kun:LabNotes/ExonomeSeq/2007-12-16|Exon capture with E55kv1 probes]]|||||||| |- | Dec-17-2007||||[[Kun:LabNotes/ExonomeSeq/2007-12-17|Another probe prep for E55kv1 set]]|||||| |- | Dec-19-2007||[[Kun:LabNotes/ExonomeSeq/2007-12-19|PCR on the captured exons (E55kv1); PAGE size selection]]|||||||| |- | Dec-20-2007||[[Kun:LabNotes/ExonomeSeq/2007-12-20|PCR cloning]]|||||||| |- | Dec-21-2007||||[[Kun:LabNotes/ExonomeSeq/2007-12-21|Exon capture with E55kv1 probes]]|||||| |- | Dec-23-2007||||[[Kun:LabNotes/ExonomeSeq/2007-12-23|Exp.1 PCR on the capturing reaction started on 12/21|PCR on captured exons (12/21/07); purified 200bp, 250bp, 350bp, 450bp bands]]|||||| |- | Dec-30-2007||||[[Kun:LabNotes/ExonomeSeq/2007-12-30|Blunt-end cloning]]|||||| |- | Dec-31-2007||||[[Kun:LabNotes/ExonomeSeq/2007-12-31|colony PCR]]|||||| |- | Jan-04-2008||||[[Kun:LabNotes/ExonomeSeq/2008-1-4|colony PCR]]|||||| |- | Jan-06-2008||||||[[Kun:LabNotes/ExonomeSeq/2008-1-6|PAGE purification of E55kv1]]|||| |- | Jan-08-2008||||||[[Kun:LabNotes/ExonomeSeq/2008-1-8|exon capture with the #P probe]]||[[Kun:LabNotes/ExonomeSeq/2008-1-8|2nd PCR on the PAGE purified 450bp band, make sequencing libs]]||[[Kun:LabNotes/ExonomeSeq/2008-1-8|Probe prep for E55kv1 and CES22k#5]] |- | Jan-09-2008||||[[Kun:LabNotes/ExonomeSeq/2008-1-9|submitted PCR amplicons to GeneWiz]]||||[[Kun:LabNotes/ExonomeSeq/2008-1-9|Sequencing lib construction (Mme I cut, PAGE purification)]]|| |- | Jan-13-2008||||||[[Kun:LabNotes/ExonomeSeq/2008-1-13|PCR on capture exon, did not work; Another round of exon capture with #P probes]]|||| |- | Jan-14-2008||||||||[[Kun:LabNotes/ExonomeSeq/2008-1-14|Sequencing lib construction (adding sequencing adaptors, PCR)]]|| |- | Jan-15-2008||||||[[Kun:LabNotes/ExonomeSeq/2008-1-15|PCR on captured exon, still did not work]]|||| |- | Jan-16-2008||||||[[Kun:LabNotes/ExonomeSeq/2008-1-16|Another round of exon capture with #P and #NP probes]]|||| |- | Jan-17-2008||||[[Kun:LabNotes/ExonomeSeq/2008-1-17|2nd PCR on the PAGE purified 450bp band]]|||||| |- | Jan-18-2008||||[[Kun:LabNotes/ExonomeSeq/2008-1-18|Sequencing library construction]]||[[Kun:LabNotes/ExonomeSeq/2008-1-18|PCR on the captured exon, only the one with #NP probes worked]]|||| |- | Jan-21-2008||||[[Kun:LabNotes/ExonomeSeq/2008-1-21|Library construction (cont.)]]||[[Kun:LabNotes/ExonomeSeq/2008-1-21|2nd PCR on captured exon, and PAGE purified 500bp+ DNA]]|||| |- | Jan-24-2008||||||[[Kun:LabNotes/ExonomeSeq/2008-1-24|Library construction (Mme I, USER/End repair/ligation or SolN6 synthesis; PCR)]]||||[[Kun:LabNotes/ExonomeSeq/2008-1-24|Probe prep for Cosmic 3760, CpG28k, CES27k sets]] |- | Jan-26-2008||||||||||[[Kun:LabNotes/ExonomeSeq/2008-1-26|Exon capture with Cosmic3760]] |- | Jan-27-2008||||||||||[[Kun:LabNotes/ExonomeSeq/2008-1-27|Another exon capture with Cosmic3760]] |- | Jan-28-2008||||||||||[[Kun:LabNotes/ExonomeSeq/2008-1-28|PCR on the 01/26/08 captured exons]] |- | Jan-29-2008||||||||||[[Kun:LabNotes/ExonomeSeq/2008-1-29|PCR on the 01/27/08 captured exons]] |- | Jan-30-2008||||||||||[[Kun:LabNotes/ExonomeSeq/2008-1-30|Library construction on the 01/29/08 amplicons]] |- | Feb-05-2008||||||||||[[Kun:LabNotes/ExonomeSeq/2008-2-5|CloneWell and PAGE purification]] |- | Feb-08-2008||||||||||[[Kun:LabNotes/ExonomeSeq/2008-2-8|Submitted 3 Solexa libs to Bing Ren's lab for sequencing]] |- | |}
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