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Continued from: http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-6-26 ===Ethanol Precipitation after PAGE size selection=== *Spun 8 tubes in -80C at 10,000rpm at 4C for 30min *Discarded supernatant and added 650ul 75% EtOH to wash and spin at 14,000rpm at 4C for 5 min *Discarded supernatant and let dry in hood 10 min *Resuspended each of the 8 tubes with 10ul H2O (80ul total) ===TBU Gel Quantification Plan=== *Dilute 2ul into 20ul *Mix 1:1 with 2X buffer (20X dilution) *Load sample wells with 4,6,8,10,12 ul *Load Low Mass DNA ladders in 0.5, 1, and 2 ul {| {{table}} | align="center" style="background:#f0f0f0;"|'''''' | align="center" style="background:#f0f0f0;"|'''0.5 Ladder''' | align="center" style="background:#f0f0f0;"|'''1 Ladder''' | align="center" style="background:#f0f0f0;"|'''4 ul''' | align="center" style="background:#f0f0f0;"|'''6 ul''' | align="center" style="background:#f0f0f0;"|'''8 ul''' | align="center" style="background:#f0f0f0;"|'''10 ul''' | align="center" style="background:#f0f0f0;"|'''12 ul''' | align="center" style="background:#f0f0f0;"|'''2 Ladder''' |- | DNA volume||0.5||1||0.2||0.3||0.4||0.5||0.6||2 |- | H2O||1.5||1||1.8||2.7||3.6||4.5||5.4||0 |- | 2X Buffer||2||2||2||3||4||5||6||2 |- | Total||4||4||4||6||8||10||12||4 |- | |} [[File:2013-06-27_0-gap-probes_GelQuantification.jpg | 650px]] *20X diluated sample concentration is 1.42 ng/ul *Starting sample concentration: 28.4 ng/ul -> total yield about 2.2ug/(193nt*325 Da/nt) = 35.1 pmole Continued on: http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-6-28
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