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==Screening 32 wells for Ampligase Positive Control Test== *Using 32 wells of 96-well plate [[Matt:LabNotes/2014-1-24]] *Used microscope to check cell density and morphology in each well to pick the best wells for this experiment **Color coded each well based on quality of cells **Designated 2 or 4 wells for each sample group ***All wells will have 0.5 U Ampligase/ul except for 2 Fixed Template wells [[File:32wellSamples.JPG]] ===Mark & Find Pos -> Well=== *E6 : Pos 1 *F6 : Pos 2 *F7 : Pos 3 *F8 : Pos 4 *F9 : Pos 5 *F10 : Pos 6 *C7 : Pos 7 *C8 : Pos 8 *C9 : Pos 9 *C10 : Pos 10 *D7 : Pos 11 *E7 : Pos 12 ===Checking MALAT1 Targets in 1000nt RT_RAB7A=== *In wells: F9 and F10 *Check with 1uM MALAT1_1stRolonyFISH (Cy3) dye-probe ===Checking for non-specific binding of dcProbe2RevComp-Cy3=== *In wells: F7 and F8 *Check with 1uM dcProbe2RevComp-Cy3 (ATTO488) dye-probe ====Result==== *No signal -> Dye doesn't have non-specific binding to primary rolonies ===Checking MALAT1, RAB7A, and ACTB Targets in RandomHex Primary Rolonies=== *In wells: E6 and F6 *Check with 1uM: **MALAT1_1stRolonyFISH (Cy3) dye-probe **RAB7A_1stRolonyFISH (ATTO550) dye-probe **ACTB_1stRolonyFISH (ATTO488) dye-probe
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