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=Test PA Gel Polymerization Temp on Swelling= *Justin read that the temperature during polymerization can affect amount of swelling when hydrogel is subjected to high temperature incubations in aqueous solution *Used [[Matt:LabNotes/2016-2-18|this gel mix]] *He did polymerizatino of one dish at room temp and the other at 4C *Then put 200ul of 50mM Tris buffer in each and incubated overnight at 37C **Buffer similar to RT buffer: [https://www.neb.com/products/b0253-m-mulv-reverse-transcriptase-reaction-buffer] [[File:IMG_010.JPG|450px]] ==Conclusion== *Polymerizing at 4C may have decreased swelling slightly but not enough to be a solution for DARTFISH *Swelling may be prevented by covalently linking matrix to glass surface
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