Editing
Matt:LabNotes/2017-1-13
Jump to navigation
Jump to search
Warning:
You are not logged in. Your IP address will be publicly visible if you make any edits. If you
log in
or
create an account
, your edits will be attributed to your username, along with other benefits.
Anti-spam check. Do
not
fill this in!
[[Matt:LabNotes/2016-8-3|Sample 2 was decoded successfully but not stained with Anti-NeuN to confirm neurons]] ==Anti-NeuN Staining Procedure== #Strip with 80% formamide twice and washing with 1X PBS #Rinse tissue with 1X PBS for 30min #Incubate section for 30min in 1ml blocking buffer #*50ul DKS + 250ul 10% BSA + 0.1ul TX-100 + 100ul 10X PBS + 600ul H2O #Incubate section for 1hr in 1ml NeuN antibody solution #*1ul rabbit anti-NeuN (Millipore MABN140) diluted in 999ul blocking buffer same as in step 2 #Wash 3x 5min in 1X PBS #Stain with Donkey anti-Rabbit Alexa 488 for 1.5hr #*DxR488 stock was diluted 1:200 in blocking buffer without the TX-100 #Wash 3x 5min in 1X PBS #*2nd wash use 5uM DRAQ5 in 1X PBS #*3rd wash use 1X PBS + 0.1% Tween-20 #Add 150ul Vectashield mounting media with antifade and allow 10min before imaging #DRAQ5 signal was very weak so: incubate in another 5uM DRAQ5 + 1X PBS for 15min and don't wash before imaging again
Summary:
Please note that all contributions to ZhangLabWiki may be edited, altered, or removed by other contributors. If you do not want your writing to be edited mercilessly, then do not submit it here.
You are also promising us that you wrote this yourself, or copied it from a public domain or similar free resource (see
ZhangLabWiki:Copyrights
for details).
Do not submit copyrighted work without permission!
Cancel
Editing help
(opens in new window)
Navigation menu
Personal tools
Not logged in
Talk
Contributions
Create account
Log in
Namespaces
Page
Discussion
English
Views
Read
Edit
View history
More
Search
Navigation
Main Page
Current events
Recent changes
Random page
Investigators
Matt Cai
Song Chen
Eric Chu
Dinh Diep
Elizabeth Duong
Shicheng Guo
Alan Fung
Daniel Jacobsen
Blue Lake
Huy Lam
Alice Li
Andrew Richards
Brandon Sos
Chris Wei
Yan Wu
Kun Zhang
Tools
What links here
Related changes
Special pages
Page information