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Rui:LabNotes/SingleCell/2011-5-17
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==Sequence result== Continue with lab note on 5.16.11 ===RNA sample Hues54=== * 15 colonies directly for TempliPhi, roughly ~500ng/ul in 10ul PCR, 3ul sending for sequencing (M13R provided by Genewiz) * No priming: 3 * mRNA: 11 * Potential non-coding region: 1 RNA-1: poor quality; first 60bp match to intergenic region with 88% >RNA-1-M13R_F01.ab1 NNNNNNANNNTGNNNNNNGCTCGGATCCNCTAGTAACGGCCGCCNGTGTGCTGGAATTCGGCTTTAATACGACTCACNNN NGGNGTATGCTTTTTTTTTTTTTTTTTTTTTTTTTCCNCNGGAANAANTGCACCTGTGTCGCCNTCCCACAGGGGAACTC GGGAGACAAAGGCGCTTTGNCNCNTCTGGGGGANCCTATCAGGACCAATTCCCATGGCGAATACCATGATATCGTCCGTN ACTGGGGGGANANTGACNTGGNTCCNAANACTGNGGNTCGGCCCGATNTTTANAAAAAAACNAACGCTAAANAAAATTGA CAATTCCAAATTAACCGGTTCCCCGTAGANCTTTCGGGGGNGGGGANCNGGGGANNNNNNNGGNCTTTCCNNCNNCCANA ANAAAAAAGAAAGGGACGGGCCGGGGAAAGGCCGGNGAAACAGGCNATAGNNCCTAGGACANTGNNNATGATNAAGACCG ACTAATGGGGGCTGCGGNCCCGTGTGTANGNGTCAGGCTGTTTCTTTTGGNANGAAGAGGGGTGGTCTTTCATANAATTC GGTGGGGGGTGGGCTCGGAGAGGGTCTACTTTGTGGAGAGNATGANGGCCAANNNANNCCNAAAGCCNAGCACNTCGGCG AAGATCAGAATCCGANNTACCNNTN RNA-2: "poor quality"; spliced form of RPL35, 98.1% >RNA-2-M13R_F02.ab1 NNNNNNNNNNNNNNNNNNNNNCTCGGANCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTCACT ATAGGGGTATCCTTTTTTTTTTTTTTTTTTTTTTTTGGAGCTGCTGAAACAGCTGGACGACCTGAAGGTGGAGCTGTCCN NNCTGCGCGTCGCCAAAGTGACAGGGGGNGCGGCCTCCAAGCTCTCTAAGATCCGAGTCGTCCGGAAATCCATTGCCCGT GTTCTCACAGTTATTAACCAGACTCAGAAAGAAAACCTCAGGAAATTCTACAAGGGCAAGTAGTACAAGCCCCTGGACCT GCGGCCTAAGAAGAAACGTGCCATGCNCCGCCGGCTCAATAANCACGANGANAACCTGAAAACCAAGAAGCAGCAGCGGA AGGAGCGGCTGTACCCGCTGCGGAAATACCCGGTCAAGGNCTGAGGGGCGCATTGTCAATAAAGCACAGCTGGNTGAGAA A RNA-3: "poor quality; spliced form of BQBP1, 98.4% >RNA-3-M13R_F03.ab1 NNNNNNNNNNNNNNNNNNNNNNCTNNNNATCCNCTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTCA CTNNNGGGGGANCCTTTTTTTTTTTTTTTTTTTTTTTTTCCCNNGANANGNTCTGNCAGGGATCGAGAGCGTGGNTATGA CAAGGTAGACAGANAGAGAGAGCGANNGNNNNAACGGGATCGGGACCGCGGGTATGANAAGGCAGACCGGGAANAGGGCA AAGAAGGGCGCCCCCATCGCCGGGAGGAGCNGNCTCCCTATCCCAAGAGCNAGAAGGCAGTAAGCCGAAAGGATGAAGAG TTANACCCCNTGGACCCTAGCTCATACNCNNACGCCCCCCGGGGCNGTNGNCAACAGGANTCCCCAAGNGGAATGAGGCN AAAAN RNA-4: PQ; MTF1, 98.6% >RNA-4-M13R_F04.ab1 NNNNNNNNNNNNNNNNNNNNNTCGGATCCNCTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTCACTA NAGGGGNNNCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAGCCGACAAGCAGAGACTCCTTCAGACCCTCAAAC AGAAACATTAAGTGCCATGGATGTGTNAGAGTTTCTNTCCCTCCAGAGCCTGGACACCCCGTCCAATCTGATTCCCATTG AAGCACTACTGCAGGGGGAGGAGGAGATGGGCCTCACCAGCAGCTTCTCCAAGTNAAGGGCCCATGTGTGCTCCCCTCTG GGAAAAGCGGGTGAGCAGGAGGCATGAGGTACAATGCCTGCCATCATGGGTCAAAAATTTGAAGGATGAAAAAATCTACT GTTTGAAATCCTCACCTTTTAGACGTATTTTTTTTATTCAN RNA-5: PQ; SNX9, 99.5% >RNA-5-M13R_F05.ab1 NNNNNNNNNNNNNNNNNCNNANNNNNNNNNCACNANTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTCAC TATANNGNGNGNNTTTTTTTTTTTTTTTTTTTTTTTAACAGGNNCNNGNNACACCGTCAGCTCGAACTCAACACTGGCAG CCACCGTCTCACCCCTGCGGAGGAGCGCTCCCGTCTCCCACAGGTGCCTTACCGCGTTCCCTCCCGCTGCTTTCATTTTT CTGACCTAATAATTACGGGAAANGGAAAGTCTGGGCCANCANCNATAAAATGACACCAAAAATAAGTANANGANATCGAA T RNA-6: no priming RNA-7:PQ; CR594125, 94.6% >RNA-7-M13R_F07.ab1 NNNNNNNNNNNNTGNNNNNAGCTCGNATNNNNTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTCACT ANNGGGGNNNCCNTTTTTTTTTTTTTTTTTTTTTTTTTTGCTTCCCCGTGGTTTAATATGAACAGAGTTGAATATGACAT TGTCTGACAAAAGAATGAACAATTTGCTGAATAAAAGCCCCAAGTCAGGATATATACACAGAAGAAATGGAGCCTCAGAG TCTCAGCACTTGTGCNCAATGAAAGGAGAAATCTTTAAAAAAAAAAATCTGTAACAGAGGATAAAAATCAAAAGGCAGCA AGGACACCGACACTGGNGAGGTGGGAAAGGGGCTNNTCANCTACCAGCATGGGCTCNNCNGCTAGAGAGCTAACCNATNT CAAGGATTTTCAACNCTGAGGTGGGTTCCANNTCCCCTGNN RNA-8: spliced NUCKS1, 99.7% >RNA-8-M13R_F08.ab1 NNNNNNNNNNNNNNNNNNNNNNTCGGNNCCNCTAGTNNCGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTCACT ATAGGGGTATCCTTTTTTTTTTTTTTTTTTTTTTTTCGAACTGCCATAGTCACTATCGTCATCATCTTCCATTAGGAAAT CTTCATCGCTGCCGGAATTCTCCTGGAATGGTGCCTCATCCTCCTCTTCTTGTTCTTCCTCACTGCCCACATCTTCCATG AGCATCTCTCTCTGTTTAGAAGCTGCTTTCGATGCCGCCTGCCGTTGTTGGCGCACATTTTTATGATCTTCTTTTTCATC TTCACTATCCTCTGCTGAGTGAGAATCATCCTTCTTGGTCTTCACATCTTTGTCTTCTGAGTCCTCACTATCTTCCTGTG AATTCTTTCCAGATCGCCTCTTATTTTTAGCTTCTCGGGGAGATGATCGAATTTTCTTAGTGGGAGGGCCCGAATCTCTT CCATAATCTTCATCTGCNTCATCAGATTCCTGAAACTGTGAGTAATCAACAACCTTCCTATTTCTGACAGGCCGCGACAT GTTCGCTGTCGAAACAGGACCGAGTCGAGAAGCCAAAGACCAGGACCCCCCCTACCCCGCGCGCTCGGCGCCCCACCCAA AANNNANNNAAANANAAAAGGGATACCCCTATNNNNNNTCGTATTNAAGCCNNNNNNNNGCAAGATATCCATCACACTGG NCGGCCGCTCGAGCATGCATCTAGAGGGNCCCAATTTCNCCCCTAT RNA-9: no priming RNA-10: spliced EST, 99.0% >RNA-10-M13R_F10.ab1 NNNNNNNNNNNNGNNNNNNNNNNNNCNNNNNNNCTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTCA CTATNGGGGTATCCTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTCCTAGCTCATCCTGGCTCAAAAAGCACCCCCACTGA GCACCTTGCGACCCCCACTCCTGCCCGCCAGAGAACAAACCCCCTTTGACTGTAATTTTCCTTTACCTACCCAAATCCTA TAAAACAGCTCCACCCCTATCTCCCTTCACTGACTCTCTTTTCGGACTCGGCCCCCCTGCACCCAGGTGAAATAAACAGC CATGTTGCTCACACAAAGCCTGTTTGGTGATCTCTTCACATGGATGCACATGAAATTTGGTGCTGTGACTCAAATCGGGG GACCTCCCTTGGGAGATCAATCCCCTGTACTCCTGTTCTTTGCTCCATGACAAAGATCCACCTATGACCTCAGGTCCTCA GACCAACCANCCCAAGGAACATCTCACCGATTGTAAATCAGGTGAAGTATGAAGTTATGAGGAGAAAGGAACAGAGGACA ACCACACAGAGAAAGTGTAGCTTTATCTACTAGAGCTGAAGACATTCAAATTTATGATCCAACAATTCTACTCTGAAGTG CAGTGGTGNATATACTGCAACAATGTGTACTCATGTGCCCTGAGAACTANNNNTGAAAAAAACCTTACAGCAATATGCNT AATANNNNNNAAAAAAAAAAAAAAAAAAAANGGGATNCCCCCTATNGNNGNNTCNNNNTTAAAGCCAAATTCTGGCNGAN ANTNCNTCANN RNA-11: PQ, CMTM3, 97.5% >RNA-11-M13R_F11.ab1 NNNNNNNNNNNNNNNNNNNANCNNNGATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTATACGACTCACTAT AGGGGTANCCTTTTTTTTTTTTTTTTTTTTTTTTTGTCAGTCTGGCTTAATTCATTATGGCTTTTTTTCAGGGTAAGAAA TTCACACCCAAGAGAATAAGGCTACAAATGCTCCAAACATGGGTCCAGAACTCCGGCTCTTTTGTGAGGCCTAGAGTCAC AAAAAATGCCATCTGTTAAAAAGCTGCCTTTGCATAAAAAAATATAAAAAGTTACAAAATTCCATCAAAGACAGATACAC AGTATAAATCGTTAGGTGAACACAGAAAGCTGGTCTCATCTCCTGCAGTGGCCCATAAACCTGCCCTGANGTCTGGAAAT TCACTGTCCTGAGGGTCCAGGAACTCCTGCCTGCTGGCTGGGCTCTAGGAAGAAGGAGGGTTTGGCTGGGCCTGCCCAGC TCCCCATGACACTGTCACTGTGATGCCACTGTTGTACCCTCTGTCTTCTGGACCAGTCAGTCTATGAGCTCTTCTTTGAA ACAGTCGTAGACCCAGAATGGTCATCTCACCCCCAGAANACATGGTTTCCAAGGGTGGGAGGTGTTAGTTATGGGTGCCG AGCCCCACTTCCTGGGCGACAAGGCAAAAGGTCAATTTCCCCCTGCTTCAAGAATGAAGAGGGAACTGAAAAAAAAAAAA AAAAAAAAAAAAAAGGATACCCCTAANANTGAGTCNTATTAAANCCGAATTCTGCAANAATCCATNACNANTGGNGGCNG CTCGAANNNGCATTTAGAGGGNCCCNATNTCGCCCCTATANTGAGGTCNNAATANNAANTCACTGGCCCNTCNTTTTAAC AACNNTCNNTGNANNGGGAAAAACCCTGGCNGTTANCCCAATNTTNANTCGCCCTTGNAAGCANNNNCCCCCCTTTTNCC AGNCTGGGNGTAATAACCGAAAAGNNCCCNCANCNNNNCGCCCNTTCCCNANAGTTGCGCNACCNGANANNGNNAATGGG NCNGCNCCCNGNNNNCGGCGNNNTTATANNNNNGNNNNNNNNGN RNA-12: PQ, HN1, 98.9% >RNA-12-M13R_F12.ab1 NNNNNNNNNNNNNNGNNNNNNNNNNTCGGANCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTC ACTNNNAGGNGATNNTTTTTTTTTTTTTTTTTTTTTTTTTTTACNNGTCCTAAACTACTAAACCTGCATTAAAAATTTCG GTTGGGGCGACCTCGGANCAAAACCCAACCTCCGANCAGTACNTGCTAAGACTTCACCAGTCAAAGCGAACTACTATACT CAATTGATCCAATAACTTGNCCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATTCTATTCTAGAGTCCATATCAAC AATAGGGTTTACGACCTCNATGTTGGATCAGGACATCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATT AAAGTCCTACGTGATCTGAGTTCAGACCGGAGNAATCCNNGTCGGTTTNTATCTACTTCAAATTCCTCCCTGTACGAAAG GACAAGAGAAATAAGGNCTACTTCACATAGNGCCTTCCCCCGTAAATGANATCATCTCAACTTAGTATNNTACCCACACC C RNA-13: no priming RNA-14: PQ, spliced TVC (truncated?) 87.6% >RNA-14-M13R_G02.ab1 NNNNNNNNNNNNNNNNNNNNNNTCGGNNNNNCTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTCACT ATAGGGGTATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAAAAGTACTGTGCTTTTATTAAAAAACAAAACAAAACAA AAAAAATACAATCAGGTACTGTCCAGACANGTTTGGGAAGGGAAAATCTCTTTTAAAGTCCTCAGTTTTCATCATCACCA TCACCATTATTATATTAATAATATTAATGTCCTTGAAATTTAAACAATATCACTTTTCTGGCTTCAGTTGTAGGAAATGT ACAAAACATCGAGGGTGGGGGTGGGGCAAGGGGTAACTTCCAATGACACAAGTTTCATCTTTGTGACCTCAACCAATAAC AATTGNACAGCTTTGTGTAAAACACAGCTGGCTCGGAGAATGACCATGTCCCCTGTATGGCCAGCTTGCCCCTCTCCCAT TCCCAGAGCCACCCAAGAGAAGTAAAATTCATGGTGTTGCTGTCACTAGGAGACTTTGCTCAATCGAACAAAAAAGTAGA AACTTGTAGACTGTTGTCTTTGGTGCAAAGGAAGTCGTTGCAGAGGGGCCTACCTCAGTGCCTCAGTGATCCGTGTGGTC TGCTTGGACGCCCATCAAATGCCCAAGGAGAGCTTCNNTGTNNNNNNTGGNTGTGTCGATGCTTTGGNAAAAATCCTTTC TTTTCTCTGGGGCCCCATTCCCTCTCTCACCNAATTATCCCTGGAATTTATGCCCNGCTTACGCCTGCCNTTCNANATTT CGGGAAGTTGCTGGGTNCTCAANTTTCANCTCACAAACTGGANTTGAGTACTCATGGCCCNTTNCAANNGGNAANCNAGT NTAANGCCCCTGACTGTNNATTATNGNNCCATATCTGCCCCATCAGGNNNCNCGATGACANTTCNNNAAAAAAAAAAAAA AAANNANAANAAAAANNGTNACCCCCTANNNNNAGTCTANTTTAAAGCCCNAATTCTCNGCNAAATANNNNTNNNNNNNG CNN RNA-15: spliced and truncated PCNA, 88.4% >RNA-15-M13R_G03.ab1 NNNNNNNNNNNNNNNNNNNNNNGNNTCGGANCCNCTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTC ACTANNNGGGNTCCTTTTTTTTTTTTTTTTTTTTTTTTACTTTNAAANTTGTTAACTTTATTTAAATTGTACAATATTTC TTAAACTGCATTTAGCGTCAAGATCNNNNNNGAGAATCACCAGTATTTGTAAAGCAGAAACATTCATGTTAACAAAACTA GTTTTTACTTTGGAGCTTATTCTTTAAGCAAATTATGGAGAATGGAGAGAAAAGAAAGTAGGTTATTTACAGAAGATATC TACATATTTTATTTTAATATTTAAGAATACTCAGGTACAAACTTGGTGACAGAAAAGACCTCAGGACACGCTGGCATCTC AGGAGCAATCTTCAAAGGTTTAGTTTTCTCAATTTCTAGCAATGCCTAAGATGCTTCCTCATCTTCAATCTTGGGAGCCA AATAATACTTTAAGTGTCCCATGTCAGCAATTTTATACTCTACAACAAGGGGCACATCTGCAGACATACTGAGTGTTACT GTTGNAGACAGTGGAGTGGCTTTTGTGAAAAAGTTCAGGTACCTCANAGCAAACGTTAGGTGAACAGGCTCATTCATCTC TATGGTTACCGCCTCCTCTTCCTTATCCACATTGCTTGTTTGTGACAACTNCCATGTTCCCGTTGCCAAGCTCTCCACTT GAAGAAAACTTAAAAAAAAAAAAAAAAAAAAAAGGATANCCCTANANNGAGTCNNATTTAAAGCCNAATTCTGCANATNN ===DNA samples=== * 15 colonies directly for TempliPhi, roughly ~500ng/ul in 10ul PCR, 3ul sending for sequencing (M13R provided by Genewiz) * No priming: 1 * Vector seq: 3 * Non-coding region with poly A/T: 10 DNA-1: vector seq. no A tail >DNA-1-M13R_G04.ab1 NNNNNNNNNNNNNNNNNNNNNNCTCGGATNNNCTAGTAACGGCCGCCAGTGTGCTGGAATTCTGCAGATATCCATCACAC TGNCNNCCGNNCGAGCATGCATCTAGAGGGCCCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTT ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTA ATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGACGCGCCCTGTAGCGGCG CATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCT TTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATT TAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGG TTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATC TCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAANAATGAGCTGATTTAACAAAAATT TAACGCGAATTTTAACAAAATTCAGGGCGCAAGGGCTGCTAAAGGAAGCGGAACACGTAGAAAGCCAGTCCGCAGAAACG GTGCTGACCCCGGATGAATGTCAGCTACTGGGCTATCTGGACAAGGGAAAACGCAAGCGCAAAGAGAAAGCANGTAGCTT GCAGTGGGCTTACATGGCGATAGCTAGACTGGGCGGTTTTATGGACAGCAAGCGAANCGGAATTGCCAGCTGGGGCGCCC TCTGGTAAGGTTGGGAAGCCCTGCANNACNGNNGNTCTGCNCAGNNNNGNNGNNNNNNNNNNNGNNGGNNNGGGGNANNA NNNNTCTNNAANAANNANNNGNGATGNNNGNTNNNTTTCNNATNNANNGAAANAANNNNGATTGCACCGCNGGTNNCNCC NNNNCNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNACNGGGNNNNNN DNA-2: vector; no A tail >DNA-2-M13R_G05.ab1 NNNNNNNNNNNNNNNNNNNNAGCTCGGATCCNCTAGTAACGGCCGCCAGTGTGCTGGAATTCTGCAGATATCCATCACAC TGGCGGCCGCTCGAGCATGCATCTAGAGGGCCCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTT ACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTA ATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGACGCGCCCTGTAGCGGCG CATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCT TTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATT TAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGG TTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATC TCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTNNAAAATGAGCTGATTTAACAAAAATT TAACGCGAATTTTAACAAAATTCAGGGCGCAAGGGCTGCTAAAGGAAGCGGAACACGTAGAAAGCCAGTCCGCAGAAACG GTGCTGACCCCGGATGAATGTCAGCTACTGGGCTATCTGGACAAGGGAAAACGCAAGCGCAAAGAGAAAGCNNTAGCTTG CAGTGGGCTTACATGGCGATAGCTAGACTGGCGGTTTTATGGACAGCAAGCGAACCGGAATTGCCAGCTGGGGCGCCCTC TGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCNGCCNGGATCTGATGGCGCAGGGGATCANATCT GATCAGAGACAGGATGANGNNCGTTTCCNCATGATTGAACAAGAATGGATTGCNNNNANGGTNNNNCNNNNNNTTNGGNN GNNANNGCTATNNNNNNTANNNNNN DNA-3: Intergenic region with 20bp poly A >DNA-3-M13R_G06.ab1 NNNNNNNNNNNNNNNNCCNAGCTCGGATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTCACT ATAGGGGTATCCTTTTTTTTTTTTTTTTTTTTTTTTTTCTTGAATTGTACTCCCATAATTCCCACATGTTGTGGGAGAGA GCTGGTGGGAGATAATTTGAATCATGGGGGTGGTTTCCCCCATACAGTTCTCATGGTAGTGAATAAATCTCATGAGATTG CATGGTTTTATCAGGGGTTTCTGCTTTTGTATCATCCTCGTTTTCTCTTGCCACTGCCATGTAAGAAGTGCCTTTCGGCC GGGTGCAGTAGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGTGGGTGGATCACAAGGTCAGGAGATCGATACC CTCCTGGCTAACACAGTGAGACCCCGTCTCTACTAAAAATACAAAAAATTAGCCAGGCATGGTGGTGGGCGCCTGTAGTC CCAGCTACTCAGGAGGCTGAGGCAGGAGAATGGCGTGAACCCCAGAGGCGGAGCTTGCAGTGAGCCGAGATCTCGCCACG GCACTCCAGCCTGGGTGACAGAGCGAGACTCTGTCTCAAAAAAAAAAAAAAAAAAAAAAAAGGATACCCCTNATAGTGAG TCNNNTTAAAGCCGAATTCTGCANATATCCATTCACACTGGNGGCCGCTNGAGNNTNCCNTNTAAAAGGGCCCAATTCNC CCTANANTGAGTNCGTATTAACAATTCACTGGCCGTCGTTTTACAACGNCGTGACTGGGAAAACCCTGGCGTTTCCCCAN CTTAATCCGCCTNGCANCACATCCCCCTTTCGCCAGCTGGGGNAATANCGAAANANGCCCGCACCNNTNNGCCCTTCCCA ACAGTTGCNCANCCTGAATGGNAAANGGANNGCNNCCCTTGTAGCGNNN DNA-4: intronic region with 40bp poly T >DNA-4-M13R_G07.ab1 NNNNNNNNNNNNNNNNNNNNNNNAANCTNGNNTCCNCTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGAC TCACTATAGGGGTATCCTTTTTTTTTTTTTTTTTTTTTTTTATCAGAAGCAAAACCACAAAATCCTCACTCTCAAGGAAT GCACAGGCTATTGCAGAAGCCAGGAAGAAATCTATATTATTACAGAGTACTTATCTCAGAGGGTTGTTATAAATATTAAA TGAGAGTTATTTTATGGAATACATCTGATCTAGTGCCTGGCACATAAAAAGTGTTATATAAGTATCAGCTATTGTAATTA CAGAGTGACAAATAAAAGGTCGGAGTGCTATGGTTATGGAATAGAATGATGAGAAAGGAAACTTCCAGGAGCTTAGGTGT ACTTTAAGTGCAGGTATCAAATATTGATGCTTCATTATGGCAAGGGGGCTAACAAGTTGCCAGTGTGTCACAAGTCAACA TGCAGGCAAAGATGGAAACATTTTCCAGATCTAAAATGTAGTTAAATATGTTGTACTTTGGAATTGTATATGAACTTTAT TCCCCTTTCAGAATTACAAATCTCTTAGAAAGCCATTTTATTTCTTTCTGAAACTGAGTTATGATCATCTACTATTTAGC ACAGAAATAAAAGGGAGGGATAAAATCTACCAACCCAGCAAAGACACATCTTGCTTNNNNNNNTCTTGAGGAAGGCCTTC AGCAGAATGGAAAGATGACTCTCTGTTGATTACTCTATGTTGAGAACCTTAAGATTCAGATGGTATAGGATTCCAGCTAG GCAGTTGTGAACTGTCCATTTGTCTTATGCAAGAAAGTCTCNTTCAGCTTTCTGAACTTTTCTGTATTTCACATACTCAT TTCTAGTTCATGCTCATGTTCAAAGCTTGCTAGAATAGCATGAAAAAGTTTCCAAAGTAAACTCAGCATACTCAAANNTT TTTCTAGGNTTATTATCTNNNNNNGGAAAGGTGAAAAGANAGCTTGCNTTTANTTTTAAACNCTTACNNNNNNNNAAAAT ANNNNNCNCTGNCANNANNNNNAAANTGACTGGNNNGNGNNCNNNCNNNANCCAGNNNTTGGNGCNANGNNGGCNNNNNN N DNA-5: intronic region with 30 poly A >DNA-5-M13R_G08.ab1 NNNNNNNNNNNNTNGNNNNNNNNNNCGGATNNNCTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTCA CTATANGGGTATCCTTTTTTTTTTTTTTTTTTTTTTTTACTCTTTGAGATAATACAACTGGCACAGAAAAATAAGGAGGA ACAAGAGAAAGAAAATTCCTTCAAAAGAAAGAATATATACAAAGACTCTGAGGTGGAAGAGATTGGCCTTTTCAAGAAAT TAAAAGAAAATCCACGTGACTAAAGCATAATGAGCAAAGGAAAGCCCAGTCAACAGGGGCAGATTATATAAGGTCTTGGA TAAATAGTGTCTATTTCATTCAAAGTATAATGGAAAGACACTGGAGGGTTAAAGCAAGGAAGTAAAATCATCGAATTTAC TTTTTTCTTTTTTAATTCATGAAGCTATACTGTGGAGAATGGATTATAGACGGGTTAAAGAAAAACAATGAGGCCGGGCA CAGTGGCTCATACCTATAATCCCAGCACTTTGGGAGGCCAAGGCAGGCAGATCACGAGGTCAGGAGACCAAGACCATCCT GGCCAACATGGTGAAACCCCGTCTGTACTAAAAATACAAAAATTAGCTGGGCATGGTGGCGGGTGCCTGTGATCCCAGCT ACTCAGAAGGCTGAGGCAGGAGAATCNTCTGAACCAGGGAGTCGGANATTGCAGTAAGCCGAGATCATGCCACTGCACTC CAGCTTTGGCAACAGAGTGAGACTCCCATCTCNAAAAAAAAAAAAAAAAANAAAAAAAANAAAAGGATACCCCTNTAGNG AGTCGTATTNAAGCCGAAATTCTGCANATATTCCATCACACTGGGNGG DNA-6: interginc region with 30 poly A >DNA-6-M13R_G09.ab1 NNNNNNNNNNNNNNNNNNNNAGCTCGNNNCCNCTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTCAC TATAGGGGTATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTCAAAACTAAAATGAAATATTATCTCCCCCCAGTTAAAATG GCTTTTATCCAAAAAACAGGCAATAACGAACACTAGAGAGGATGTGAAGAAAAGGGAACCCTCGTACACTGTTGTTGGGA ATGTAAATTAAGACAACCACTATAGAGAACAGTTTGGAGGTTCCTCAAAAAACTAAAAATAGGGGTCCAGCACGGTGGCT CACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGCGGGCGGCTCACAAAGTTAGGAGATCGAAACCATCCTGGTTGACA CAGTGAAACCCCGTCTCTACCAAAAACACAAAAAATTAGCTGGGCATGGTGGCAGGTCCCTGTAGTCCCAGCGACTCAGG AGGCTGAGGCAGGAGAATGGGGTGGACCCGGGAGGCAGAGCTTGCAGTAAGCTGAGATCACGCCACTGCACTCCAGCCTA GGCGACAGAGCGAGACTTGGTCTCAAAAAAAAAAAAAAAAAAAAAAAAGGATACCCCTATAGTGAGTCGNATTAAAGCCN AATTCTGNNAANATCCNTNACACTGGCGGCCGCTCGAGCATGCATCAAAAGGNNNCNAANTCGCCCTATNNNGANTCGNA TTNCANNTCACTGGCCGTCGTTTTACAACGTCNNACTGGNAAAACCCCTGGCGTTACCNAACTTAANNNNCTTGCAGCAC ATNCCCCCTTTCCNCCNGNTGGCGTTATANCGNAGAGGCCCNGCACCGGATCNCCCNTTCCCAANANTTNGCNNCNACCC TG DNA-7: interginc with 15 poly T >DNA-7-M13R_G10.ab1 NNNNNNNNNNNNNNNNNNNNNNNNNTCNNNNCCNNTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTC ACNANNGGGGTATCCTTTTTTTTTTTTTTTTTTTTTTTTTGCTTTAAGTATATTTTAAATATCCTAAACAAAATATTAGC AAAGTGAATCTAACAAACATACTTAACATTTTGTTGAGAATATTCTTATAGAAAGAATAATACACCACAAACAAGTGGGA TTTATTTCAGGGATGCAAGGCTGGTTCAACATTTAAAAATCAGTTAATGTTATCCATCACACCAACAAGCTAAAAAGGAA CAGTCATATATTCATATTAATAAATGAAGAAAAAGCAGTTGAAAAAATTCAACATTCAAGATTTAAAAAACAACAAATTA GGAGAGGACTTCCTCGACTTGTTAAGGAACATCTATAAAAAACCTATAGCTAACATCATATATAATGGTGAGAAACACAA ATCTCTTACATTAAGATCAGGGAAAAAGCAAGACTGTTCTCTGTCCCCACAGCTTTTCAACATTATATAGGAAGTTTAAG CTAATGCAATGAAAAAGAAAAGAAAAGGTAGATAAATTGGAAAAGGAGAAATTAAACTGTCTTTGTTTTCAGGTGACATA TTTACCTATGGAGAATATCCAAAGAAGCTGACGAGAAAAAAAACCTGAAACCCATAAGCAATTAGGTTTCAGGATAAACG GTTAATGTACAAAAGACAATTACTTTTTTATGCACCATCAATGGAAAATGAGACTTGAATTGTAAAATACAGTATCACTC ATATTAGCACCCAAAAATGAATAGAAANTAAATCAAGCAACATATGTACNAGATCTATATGAGAAAAACTATAAAAATCT GANCAAAAATATATCTAAGAAGAANCTAAATAAANNAAGAGAGTCCATGNTTCATGGANNAGAAANGACTAAANATNGTC AACTTGTCAGTTTCCNTNCCNNACTTGATCTACAGACNTNCACAAAAATCCTNCAGTCNAAAACCNNNCANCAAANTNTT NNNNNNNNNNNNNCCNNNNNAANNTGGATNCCNNNANNNNNATNNNNNNNAANANNCNGNANNNGCTNATNCNANACTNA AANNNNNGNAAATNNNNNNNTNNNN DNA-8: intronic region, 16 poly A >DNA-8-M13R_G11.ab1 NNNNNNNNNNNNNNNNNNNNGCTCGGATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTCACT ATAGGGGTATCCTTTTTTTTTTTTTTTTTTTTTTTTCGGCATTTCGGAGGCCTAGGTGGGTGGATCACTTGAGGTCAGGA GTTCAAGACCAGTCTGGCCAACATGGTGAAACCCCATCNCNACTAAAAATACAAAAATTAGCAGGGCGTGGTGGCAGGCC CTTGTAATCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATCCCTTGAACCTGGGAGGCGGAGGTTGCAGTAAGCTGAGAT TGCACCACTGAACTCCAGCCTGGCTGATAAAACAAGACTTCAACTAAAAAAAAAAAAAAAAAAANAAAAGGATACCCCAA TA DNA-9: intronic with 25 poly T >DNA-9-M13R_G12.ab1 NNNNNNNNNNNNNNNNNNNNNNCNNGNATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTCAC TATAGGGGTATCCTTTTTTTTTTTTTTTTTTTTTTTTTTGAGACAGTCTNGGTCTGTTGCCCAGGCTGGAGCACANCGGC NTGATCCTGGCTCACTGCAGCCTCCACCTCTGAGGTTCAGCAATTCTCCTGCTTNNGCTTGAGTAGCTGGTACTACAGGN ACATGCCACCATGCCCAGCTAATTTTTGTATTTTTGGTAAAGACAGGGTTTCGCCATGTTGGCCAGTCTGGTCTTGAANN CCTGACTTCAAGTGATCCACCAGCCTCAGCCTCCCAAAGTGG DNA-10: no priming DNA-11: intergenic with 22 poly A >DNA-11-M13R_H02.ab1 NNNNNNNNNNNNNNNNNNNNNNNGNNTCGNNNNNCACTAGTAACGGCCCGCNNAGTGTGNTGGAATTCGGCTTTAATACG ACTCACTNNNGNTNNTNTCCTTTTTTTTTTTTTTTTTTTTTTTTTATCTTCTGTTATAGTCACAATAACATGTTATGCCT TTGCTTGTATGTTCATCTCTCTCTGTGTACTAGCAGTCCATCCTAATAATGATCTTATCATCTGGAATATAATTGAATAT TACAATTGAGAATAAAATAGATTATCCAGATTATTTCAGTGAATATAAGTCTAGCTGACTAAGAGGGAAAAATAATTATA GATACCGTGACATCCCCACACCAACTTCTTGTAACAATGAGATGAGTGAAACTTTAATGAAACAGTGTTATCATCAAAGA CCTGCCAACCTAAAATTATCTGTTTTGATGAATTGTCATTAAATAAATGAAGACGCCAAAATAAAGATATTTTATACAAA AACTAAATCCCTTATTCCCTCAGAGAGTTTGTAGGTTTCATTCCAAAAAGCCACAATAAAGTGAATATTGCAATAAAGCA AGTCACACAAATTTTTTGGTTTCCCAGTGCATATAAAAGTTATGCTTGGGGCTGGGCACGGTGGCTCACGCCTGTAATCC CAGCTCTTTGGGAGGCCGAGGCAGGCGGATCACAAGGTCNNNNGATCGAGACCATCCTGGCTAACACGGTGAAACCCCGT CTCTATTGAAAATACAAAAAATTAGTCGGGCGCAGTGGCGGGCGNCTGTAGTCCCAGCTACTGGGGAGGCTGAGGCAGGA NAATGGTGNCAACCNGGGGGGCGGGAGCTTGCAGTGAGCCGAGATTGCGCCACTGCACTCCAGCCTGGTGACAGANCGAG ACTGCNTCTCNAAANAAAAANAAANNANAAAAANGNNACCCCNATAGNNGNGTCGTATAAANNCNNATTCTGCNNATNTC ATTNNCANNNNCGGNCNGCTCNAGCATGCATCTAGAGGGCCNAATTNNNCCTNTANNNAGTCGTATTANATTNACTGGCN GTNNTTTTANNANNGTNNNNGACNNNGGAAAANCCTGNNN DNA-12: intergenic with 23 poly T >DNA-12-M13R_H03.ab1 NNNNNNNNNNNNTNGNNNNNNNNNTNGNNNNNNNANTANCGGCCGCCNGTGTGCTGGAATTCGGCTTTAATACGACTCAN NNTANNGGNNNNNNTTTTTTTTTTTTTTTTTTTTTTTTGANACAAGAGTNTCCCTCTGTCATCCAGGCTGGAGTGCAGTG GCGTGATGTTGGCTCACTGCAACCTCTGCCTGCCAGGTTCAAGCAATTCTCCTGCCTCAGCCTTCTGAGTAGCTAGGATT GCAAGCACATACCACCATACCCGGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCACCATGTTGGCCAGGATGGTCT CAATCTCTTGACCTCGGGATCTGCCCGCCTTGGCCTCCCAAAGTGCTGGGATTACACGCNTGAGCCACCATGCATGGCCA AAAGCTGCTACNTCTAAAGGTGTTTCTTAGAAATAGCAAAGNANTATAGCCAGGAAAAACAATTACAACAGCCACAAACT CTAN DNA-13: vector seq. >DNA-13-M13R_H04.ab1 NNNNNNNNNNNNNNNNNNNNNNNNCTCNNNNNNNCTAGTAACGGCCGCCAGTGTGCTGGAATTCTGCAGATATCCATCAC ACTGGCGGCCGCTCGAGCATGCATCTAGAGGGCCCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTT TTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGACGCGCCCTGTAGCGG CGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCG CTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGA TTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGAC GGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTA TCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCNNTTGGTTAAAAAATGAGCTGATTTAACAAAAA TTTAACGCGAATTTTAACAAAATTCAGGGCGCAAGGGCTGCTAAAGGAAGCGGAACACGTAGAAAGCCAGTCCGCAGAAA CGGTGCTGACCCCGGATGAATGTCAGCTACTGGGCTATCTGGACAAGGGAAAACGCAAGCGCAAAGAGAAAGCANGTAGC TTGCAGTGGGCTTACATGGCGATAGCTAGACTGGGCNGGTTTTATGGACAGCAAGCGAAACCGGAATTGCCAGCTGGGGC GCCCTCTGNTAAGGTGGGAAGCCCTGCAAAGTAAACTGGAATNGCTTCTTGCCGCCAAGGATCTGATGGCGCANGGGATC AAGAANCTGATCAANNANANNGNATGANGNATCGTTTCGCATGAATGAANAANAATGGAATTGCNCNCAGGNTNNNCNGN N DNA-14: intergenic region with 16 poly T/G >DNA-14-M13R_H05.ab1 NNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNTCCACTAGTAACGGCCGCNAGTGTGCTGGAATTCGGCTTTAATACGA CTCACTATNGGGGNNTCCNTTTTTTTTNTTTTTTTTTTTTTTGAGACAGAGTCTCGCTCTGTCCCCAAGTTGGAGTGNNN TGGCCCGN DNA-15: intergenic region >DNA-15-M13R_H06.ab1 NNNNNNNNNNNNNNNNACNAGNNNGGANCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTTAATACGACTCNCN NNANNGNNNNGCNTTTTTTTTTTTTTTTTTTTTTTTCTCNGCNANNACCNNNAAATATCATGGTCTCTGGCAAGATGTCC AAAATCTGATTCAGGTCTTCCGGTCAGTCGTAGCCAGCTATCA
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