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Sam:LabNotes/Microbiome-new/2009-2-23
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=Plate counging for diluted E. coli used in previous MDA exp= ==Objective== * To confirm the bacterai number in previous Bac MDA experiment ==Procedures== *Prepare the non-antibiotics LB plates *Plating ** No.1 (1000 cell/uL) -> 1/10 dilution -> 50 uL plating on w/o antibiotics LB agar plates x 2 duplicates ** No.2 ( 100 cell/uL) -> 1/10 dilution -> 50 uL plating on w/o antibiotics LB agar plates x 2 duplicates ** No.3 ( 10 cell/uL) -> 1/10 dilution -> 50 uL plating on w/o antibiotics LB agar plates x 2 duplicates *Incubate at 37 degree incubator over night. *Count the plate the next day. ==Results== *Expected number of plate counting **No.1 1/10 diluted 1000 cell/uL x 50 uL = 5000 cell --- not countable **No.2 1/10 diluted 100 cell/uL x 50 uL = 500 cell --- not countable **No.3 1/10 diluted 10 cell/uL x 50 uL = 50 cell --- 53 cells (Replicate 1) and 79 cells (Replicates 2) *The results showed that OD600 = 1 -> 10^9 CUF/mL of E. coli is a good estimation.
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