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Tina:System operation
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*'''Components:''' 1. microfluidic system 2. microincubator controller 3. microincubator manifold 4. 5% pre-mixed CO2 tank *'''System operation:''' 1. Attach the luer fittings from the "microincubator manifold" to the front panel of the "microfluidic system" and the "microincubator controller" in correct order (color matching). 2. Turn on "microfluidic system". Power indicater (red) and system ready light (blue) should be on. 3. Turn on "microincubator controller" if temperature and CO2 control is needed. Temperature display will show current temperature. Gas flow light (green) will be on once introduce the gas flow. 4. Turn the pressure adjusting knob of the CO2 tank. Make sure the gas flow is 45-55 psi. Check the remaining gas amount in tank. (Approximately usage is ~100 psi/24 hrs experiment.) 5. Open the CellASIC ONIX FG software on desktop.Select the plate type you are using. (Usually, mammalian M04S. For open top plate, select M04L.) 6. Make sure the status indicator on the bottom of the window says the system is ready. 7. Seal the manifold to the plate. Wipe the manifold clean with 70% EtOH (use Kimwipes). Align the manifold to the plate, and turn the vacuum on (Keep pushing down during sealing!). When the plate is properly sealed, the seal light (green) on the "microfluidic system" will be on. Sometimes it could be really difficult to seal a plate, if the plate is new. The system ready light (blue) might turn off during sealing. If this happens, turn the vacuum off and wait for the ready light to turn on again. Then, turn the vacuum back on. Repeat the process until plate is sealed. Don't let the vacuum be on while the ready light is off. Otherwise, the system would be over-heated and automatically shut down. 8. Place the sealed plate on microscope. 9. Go back to the software. Create protocols. Save it and run.
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