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Transformation Protocol
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#Ensure water bath at 42 C, warm SOC media in incubator at 37 C #Add 200 ng DNA into each tube of 100 uL cells (1-5 uL vector). You want to use up all of your vector so as to get the best coverage. #Incubate on ice for 30 minutes #Heat-shock for 45 seconds @ 42 C #Incubate on ice again for 2 minutes #Rescue cells with 500 uL SOC per tube #Shake at 37 C for 1 hr in incubator #Spread up to 20 uL on at least 2 plates for each library. For control spread 100 uL on each plate, using at least 2 plates. #Add all tubes into large culture flask (150 mL) grow overnight #The next day, make at least 10 tubes of frozen stock from large culture and then maxi-prep
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