Passaging mESCs on feeder: Revision history

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7 May 2013

  • curprev 22:2122:21, 7 May 2013>TinaLo 279 bytes +279 Created page with "1. Aspirate media from cells 2. Wash well once with PBS 3. Add 1ml TrpLE to each well, incubate 5 min at 37C 4. Remove TrpLE 5. Add 1ml mESC media 6. Pipette using p1000..."