Kun:LabNotes/ASE/2007-12-31: Difference between revisions
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10x buffer 3ul 3ul 3ul | 10x buffer 3ul 3ul 3ul | ||
H2O (DEPC) 17ul 10ul 0ul | H2O (DEPC) 17ul 10ul 0ul | ||
DNase I(2u/ul) 2ul 2ul 2ul | |||
RNase Inhibitor 0.5ul 0.5ul 0.5ul | |||
37C 30min -> 75C 10min -> Cleanup with RNeasy columns | |||
==Exp.2 Also process RNAs from Hybrid1, Hybrid2, BJ Fibroblast== | |||
Hybrid1 Hybrid2 BJ Fibroblast | |||
total RNA 1.9ug/ul x8ul 570ng/ul x 20ul 72ng/ul x 52ul | |||
10x buffer 3ul 3ul 6ul | |||
H2O (DEPC) 17ul 5ul 0ul | |||
DNase I(2u/ul) 2ul 2ul 2ul | DNase I(2u/ul) 2ul 2ul 2ul | ||
RNase Inhibitor 0.5ul 0.5ul 0.5ul | RNase Inhibitor 0.5ul 0.5ul 0.5ul | ||
37C 30min -> 75C 10min -> Cleanup with RNeasy columns | 37C 30min -> 75C 10min -> Cleanup with RNeasy columns |
Revision as of 23:24, 31 December 2007
Exp.1 Make gDNA-free cDNA from GM20431, GC1EP and Hues6
GM20431(06/04/07) GC1EP(06/14/07) Hues6(05/14/07) total RNA 1.25ug/ul x8ul 645ng/ul x 15ul 270ng/ul x 25ul 10x buffer 3ul 3ul 3ul H2O (DEPC) 17ul 10ul 0ul DNase I(2u/ul) 2ul 2ul 2ul RNase Inhibitor 0.5ul 0.5ul 0.5ul 37C 30min -> 75C 10min -> Cleanup with RNeasy columns
Exp.2 Also process RNAs from Hybrid1, Hybrid2, BJ Fibroblast
Hybrid1 Hybrid2 BJ Fibroblast total RNA 1.9ug/ul x8ul 570ng/ul x 20ul 72ng/ul x 52ul 10x buffer 3ul 3ul 6ul H2O (DEPC) 17ul 5ul 0ul DNase I(2u/ul) 2ul 2ul 2ul RNase Inhibitor 0.5ul 0.5ul 0.5ul 37C 30min -> 75C 10min -> Cleanup with RNeasy columns