AlanFung:Protocol/Qubit: Difference between revisions
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*Press <Go> | *Press <Go> | ||
*Repeat until all samples have been read | *Repeat until all samples have been read | ||
==Calculation the Concentration of your Sample== | |||
*Concentration of your sample = QF value x (200/x) | |||
*where QF = the value given by the Qubit fluorometer | |||
x = the number of microliters of sample you added to the assay tube |
Revision as of 21:49, 30 June 2009
Qubit Fluorometer Protocol
Samples & Materials
- Qubit Assay Tubes (500, Invitrogen Cat. no.Q32856) or Axygen PCR-05-C tubes (VWR, part number 10011-830)
Calibrating the Qubit Fluorometer
Running a New Calibration
- Insert standard #1
- Press <Go>
- Wait for ~5s
- Insert standard #2
- Press <Go>
Using the Last Calibration
- Highlight <Use Last Calibration>
- Press <Go>
Reading Samples
- Insert sample
- Press <Go>
- Read the result on the display
- Record the reading
- Remove the sample form the instrument
- Insert the next sample
- Press <Go>
- Repeat until all samples have been read
Calculation the Concentration of your Sample
- Concentration of your sample = QF value x (200/x)
*where QF = the value given by the Qubit fluorometer x = the number of microliters of sample you added to the assay tube