Jie:LabNotes/CpgSeq/2009-7-14: Difference between revisions

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[[Image:20090714_genomic alleotype validatioon of GLIPR1L2 on GM12863,64,72~75.jpg]]20090714_genomic alleotype validatioon of GLIPR1L2 on GM12863,64,72~75
[[Image:20090714_genomic alleotype validatioon of GLIPR1L2 on GM12863,64,72~75.jpg]]20090714_genomic alleotype validatioon of GLIPR1L2 on GM12863,64,72~75
==Sample Par. ship to Billy==
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Sample ID'''
| align="center" style="background:#f0f0f0;"|'''Concentration (ng/ul)'''
| align="center" style="background:#f0f0f0;"|'''Volume (ul)'''
| align="center" style="background:#f0f0f0;"|'''Total Amt. (ng)'''
|-
| Par_1||21.22||30||636.6
|-
| Par_2||15.25||30||457.5
|-
| Par_3||18.05||30||541.5
|-
| Par_4||19.97||30||599.1
|-
| Par_5||29.63||30||888.9
|-
| Par_6||15.82||30||474.6
|-
| Par_7||35.41||30||1062.3
|-
| Par_8||46.59||30||1397.7
|-
| Par_9||22.29||30||668.7
|-
| Par_10||30.4||30||912
|-
|
|}

Revision as of 22:35, 14 July 2009

genomic alleotype validatioon of GLIPR1L2 on GM12863,64,72~75

reaction system                                                 x6     
H2O                                                  20ul      120ul    
2x Mastermix                                         25ul      150ul      
AmpF(10uM)                                           2ul       12ul       
AmpR(10uM)                                           2ul       12ul     
template                                      1ul/each from each sample
Total                                                50ul      300ul
94C 2min -> (94C 45S -> 55C 45S -> 72C 45S) x 8 ->72C 3 min -> 15C hold.

File:20090714 genomic alleotype validatioon of GLIPR1L2 on GM12863,64,72~75.jpg20090714_genomic alleotype validatioon of GLIPR1L2 on GM12863,64,72~75