Jie:LabNotes/CpgSeq/2009-7-14: Difference between revisions

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>Zsakura2
(New page: ==genomic alleotype validatioon of GLIPR1L2 on GM12863,64,72~75== reaction system x6 H2O ...)
 
>Jie deng
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==genomic alleotype validatioon of GLIPR1L2 on GM12863,64,72~75==
==genomic alleotype validatioon of GLIPR1L2 on GM12863,64,72~75==


reaction system                                                x6     
reaction system                                                x6     
H2O                                                  20ul      120ul     
H2O                                                  20ul      120ul     
2x Mastermix                                        20ul     120ul      
2x Mastermix                                        25ul     150ul      
AmpF(25uM)                                          0.5ul       3ul        
AmpF(10uM)                                          2ul       12ul        
AmpR(25uM)                                          0.5ul       3ul      
AmpR(10uM)                                          2ul       12ul      
template                                      1ul/each from each sample
template                                      1ul/each from each sample
Total                                               40ul      240ul
Total                                               50ul      300ul
94C 2min -> (94C 45S -> 55C 45S -> 72C 45S) x 35 ->72C 3 min -> 15C hold.
 
primers:
GLIPR1L2_gDNA_imprinted_F:5' - TGT GGC TAC TGC TAC TGG GTT CTA - 3'
GLIPR1L2_gDNA_imprinted_R:5' - AGC ATG TGT TAG AGT GTC CGC AGT - 3'
 
[[Image:20090714_genomic alleotype validatioon of GLIPR1L2 on GM12863,64,72~75.jpg]]20090714_genomic alleotype validatioon of GLIPR1L2 on GM12863,64,72~75
 
I purified the samples and sent to Genewiz to do the sanger sequencing.

Latest revision as of 22:11, 25 July 2009

genomic alleotype validatioon of GLIPR1L2 on GM12863,64,72~75[edit]

reaction system                                                 x6     
H2O                                                  20ul      120ul    
2x Mastermix                                         25ul      150ul      
AmpF(10uM)                                           2ul       12ul       
AmpR(10uM)                                           2ul       12ul     
template                                      1ul/each from each sample
Total                                                50ul      300ul
94C 2min -> (94C 45S -> 55C 45S -> 72C 45S) x 35 ->72C 3 min -> 15C hold.
primers: 
GLIPR1L2_gDNA_imprinted_F:5' - TGT GGC TAC TGC TAC TGG GTT CTA - 3' 
GLIPR1L2_gDNA_imprinted_R:5' - AGC ATG TGT TAG AGT GTC CGC AGT - 3'

File:20090714 genomic alleotype validatioon of GLIPR1L2 on GM12863,64,72~75.jpg20090714_genomic alleotype validatioon of GLIPR1L2 on GM12863,64,72~75

I purified the samples and sent to Genewiz to do the sanger sequencing.