HUES: Difference between revisions

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==='''HUES Cell Collection'''===
=='''HUES Cell Collection'''==
==HUES homepage==
*HUES homepage[http://www.mcb.harvard.edu/melton/HUES/index.html]
 
==Protocol==
==Protocol==
*Protocol [[Media:hues.pdf]]
*Protocol [[Media:hues.pdf]]
==Storage==
==Storage==
*Location:Dr. Varghese lab LN tank Rack #2 Box "Zhang Lab"
*Location:CERC LN Tank
 
==HUES Cell Log==
==HUES Cell Log==
*1 x HUES-3 p22
*1 x HUES-3 p22
Line 12: Line 16:
*1 x HUES-66 p12
*1 x HUES-66 p12
*2 x PMEFi
*2 x PMEFi
==Materials and Reagents==
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Materials / Catalogue no.  '''
| align="center" style="background:#f0f0f0;"|'''Provider'''
| align="center" style="background:#f0f0f0;"|'''CAT#'''
|-
|  ||||
|-
| KO‐DMEM      || Invitrogen Gibco  ||  CAT#10829 
|-
|  ||||
|-
| DMEM        ||Invitrogen Gibco    ||CAT#11965‐092 
|-
|  ||||
|-
| FBS        ||Valley Biomedical, Inc||CAT#BS‐3033 
|-
|  ||||
|-
| ||||
|-
| KO‐Serum Replacenment ||Invitrogen Gibco    ||CAT#10828 
|-
|  ||||
|-
| Plasmanate  ||Bayer||CAT#NDC 0026‐0613‐20 
|-
|  ||||
|-
| bFGF        ||Invitrogen Gibco    ||CAT#13256‐029 
|-
|  ||||
|-
| hLIF        ||Chemicon International||CAT#LIF1010 
|-
|  ||||
|-
| 2‐beta‐mercaptoethanol||Gibco||CAT#21985‐023 
|-
|  ||||
|-
| Non‐essential amino acids (NEAA) ||Invitrogen Gibco    ||CAT#11140050 
|-
| (10mM or 100x)||||
|-
|  ||||
|-
| Pen/Strep    ||Invitrogen Gibco    ||CAT#15070‐063 
|-
| (5000U/ml Penicillin G sodium and|||| 
|-
| 5000ug/ml Streptomycin sulfate in 0.85% saline)  ||||
|-
|  ||||
|-
| Glutamax‐I  ||Invitrogen Gibco    ||CAT#35050‐061 
|-
| (200mM or 100x)  ||||
|-
|  ||||
|-
| Mitomycin C||Sigma    ||CAT#M‐0503 
|-
|  ||||
|-
| 0.05% Trypsin/EDTA||Invitrogen Gibco    ||CAT#25300‐054 
|-
|  ||||
|-
| Dimethyl sulphoxide (DMSO)||Sigma    ||CAT#D2650 
|-
|  ||||
|-
| Gelatin      ||Sigma      ||CAT#G1890 
|-
|  ||||
|-
| Tissue Culture Treated Plates (6 well)||Coring||CAT#3506
|-
|  ||||
|-
| Hygromycin resistant, pre-treated MEFs ||Specialty Media||CAT#PMEF‐H 
|-
|  ||||
|-
| Neomycin resistant, pre-treated MEFs||Specialty Media||CAT#PMEF‐N
|-
|
|}
==Contact Information==
==HUES Cell Facility Contact Information==
*HUES Facility / Melton Laboratory / HHMI
*Harvard University
*Cambridge, MA 02138
*Fax: 617.384.9463
*Please send all HUES cell correspondence to hues@mcb.harvard.edu.
==Harvard's Office of Technology Development Contact Information==
*Harvard's Office of Technology Development
*1350 Massachusetts Avenue
*Holyoke Center 727
*Cambridge, MA 02138
*Fax: 617.495.9568
*Please send all correspondence concerning the MTA to Material Transfers: materialtransfer@harvard.edu.

Latest revision as of 23:49, 20 May 2011

HUES Cell Collection[edit]

HUES homepage[edit]

  • HUES homepage[1]

Protocol[edit]

Storage[edit]

  • Location:CERC LN Tank

HUES Cell Log[edit]

  • 1 x HUES-3 p22
  • 1 x HUES-8 p19
  • 1 x HUES-53 p11
  • 1 x HUES-62 p10
  • 1 x HUES-64 p12
  • 1 x HUES-66 p12
  • 2 x PMEFi

Materials and Reagents[edit]

Materials / Catalogue no. Provider CAT#
KO‐DMEM Invitrogen Gibco CAT#10829
DMEM Invitrogen Gibco CAT#11965‐092
FBS Valley Biomedical, Inc CAT#BS‐3033
KO‐Serum Replacenment Invitrogen Gibco CAT#10828
Plasmanate Bayer CAT#NDC 0026‐0613‐20
bFGF Invitrogen Gibco CAT#13256‐029
hLIF Chemicon International CAT#LIF1010
2‐beta‐mercaptoethanol Gibco CAT#21985‐023
Non‐essential amino acids (NEAA) Invitrogen Gibco CAT#11140050
(10mM or 100x)
Pen/Strep Invitrogen Gibco CAT#15070‐063
(5000U/ml Penicillin G sodium and
5000ug/ml Streptomycin sulfate in 0.85% saline)
Glutamax‐I Invitrogen Gibco CAT#35050‐061
(200mM or 100x)
Mitomycin C Sigma CAT#M‐0503
0.05% Trypsin/EDTA Invitrogen Gibco CAT#25300‐054
Dimethyl sulphoxide (DMSO) Sigma CAT#D2650
Gelatin Sigma CAT#G1890
Tissue Culture Treated Plates (6 well) Coring CAT#3506
Hygromycin resistant, pre-treated MEFs Specialty Media CAT#PMEF‐H
Neomycin resistant, pre-treated MEFs Specialty Media CAT#PMEF‐N

Contact Information[edit]

HUES Cell Facility Contact Information[edit]

  • HUES Facility / Melton Laboratory / HHMI
  • Harvard University
  • Cambridge, MA 02138
  • Fax: 617.384.9463
  • Please send all HUES cell correspondence to hues@mcb.harvard.edu.

Harvard's Office of Technology Development Contact Information[edit]

  • Harvard's Office of Technology Development
  • 1350 Massachusetts Avenue
  • Holyoke Center 727
  • Cambridge, MA 02138
  • Fax: 617.495.9568
  • Please send all correspondence concerning the MTA to Material Transfers: materialtransfer@harvard.edu.