HUES: Difference between revisions
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*Protocol [[Media:hues.pdf]] | *Protocol [[Media:hues.pdf]] | ||
==Storage== | ==Storage== | ||
*Location: | *Location:CERC LN Tank | ||
==HUES Cell Log== | ==HUES Cell Log== | ||
*1 x HUES-3 p22 | *1 x HUES-3 p22 | ||
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{| {{table}} | {| {{table}} | ||
| align="center" style="background:#f0f0f0;"|'''Materials / Catalogue no. ''' | | align="center" style="background:#f0f0f0;"|'''Materials / Catalogue no. ''' | ||
| align="center" style="background:#f0f0f0;"|'''''' | | align="center" style="background:#f0f0f0;"|'''Provider''' | ||
| align="center" style="background:#f0f0f0;"|'''''' | | align="center" style="background:#f0f0f0;"|'''CAT#''' | ||
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| FBS | | FBS ||Valley Biomedical, Inc||CAT#BS‐3033 | ||
|- | |- | ||
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|- | |- | ||
| | | |||| | ||
|- | |- | ||
| KO‐Serum Replacenment ||Invitrogen Gibco ||CAT#10828 | | KO‐Serum Replacenment ||Invitrogen Gibco ||CAT#10828 |
Latest revision as of 23:49, 20 May 2011
HUES Cell Collection[edit]
HUES homepage[edit]
- HUES homepage[1]
Protocol[edit]
- Protocol Media:hues.pdf
Storage[edit]
- Location:CERC LN Tank
HUES Cell Log[edit]
- 1 x HUES-3 p22
- 1 x HUES-8 p19
- 1 x HUES-53 p11
- 1 x HUES-62 p10
- 1 x HUES-64 p12
- 1 x HUES-66 p12
- 2 x PMEFi
Materials and Reagents[edit]
Materials / Catalogue no. | Provider | CAT# |
KO‐DMEM | Invitrogen Gibco | CAT#10829 |
DMEM | Invitrogen Gibco | CAT#11965‐092 |
FBS | Valley Biomedical, Inc | CAT#BS‐3033 |
KO‐Serum Replacenment | Invitrogen Gibco | CAT#10828 |
Plasmanate | Bayer | CAT#NDC 0026‐0613‐20 |
bFGF | Invitrogen Gibco | CAT#13256‐029 |
hLIF | Chemicon International | CAT#LIF1010 |
2‐beta‐mercaptoethanol | Gibco | CAT#21985‐023 |
Non‐essential amino acids (NEAA) | Invitrogen Gibco | CAT#11140050 |
(10mM or 100x) | ||
Pen/Strep | Invitrogen Gibco | CAT#15070‐063 |
(5000U/ml Penicillin G sodium and | ||
5000ug/ml Streptomycin sulfate in 0.85% saline) | ||
Glutamax‐I | Invitrogen Gibco | CAT#35050‐061 |
(200mM or 100x) | ||
Mitomycin C | Sigma | CAT#M‐0503 |
0.05% Trypsin/EDTA | Invitrogen Gibco | CAT#25300‐054 |
Dimethyl sulphoxide (DMSO) | Sigma | CAT#D2650 |
Gelatin | Sigma | CAT#G1890 |
Tissue Culture Treated Plates (6 well) | Coring | CAT#3506 |
Hygromycin resistant, pre-treated MEFs | Specialty Media | CAT#PMEF‐H |
Neomycin resistant, pre-treated MEFs | Specialty Media | CAT#PMEF‐N |
Contact Information[edit]
HUES Cell Facility Contact Information[edit]
- HUES Facility / Melton Laboratory / HHMI
- Harvard University
- Cambridge, MA 02138
- Fax: 617.384.9463
- Please send all HUES cell correspondence to hues@mcb.harvard.edu.
Harvard's Office of Technology Development Contact Information[edit]
- Harvard's Office of Technology Development
- 1350 Massachusetts Avenue
- Holyoke Center 727
- Cambridge, MA 02138
- Fax: 617.495.9568
- Please send all correspondence concerning the MTA to Material Transfers: materialtransfer@harvard.edu.