Alice:LabNotes/2008-1-12: Difference between revisions
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[[Image:ZhangLab_2 2008-01-12 15hr 00min.jpeg]]<br> | [[Image:ZhangLab_2 2008-01-12 15hr 00min.jpeg]]<br> | ||
The very bottom very bright bands are the ladder for both columns (3uL ladder, 1 uL TBE buffer, and 1 uL of loading dye), and the rest of the wells contains sample 1-16 (4uL sample and 1uL of loading dye).<br> | |||
I ran a gel to see if all the reaction came out to be working. Even though the bands are not very bright for the left column (which is the first 8 samples), they are still working but just barely reached lateau. This might be caused by not well mixed tube setup. However, the gel looks fine, and the expriment can be continued. | I ran a gel to see if all the reaction came out to be working. Even though the bands are not very bright for the left column (which is the first 8 samples), they are still working but just barely reached lateau. This might be caused by not well mixed tube setup. However, the gel looks fine, and the expriment can be continued. |
Revision as of 01:59, 16 January 2008
File:ZhangLab 2 2008-01-12 15hr 00min.jpeg
The very bottom very bright bands are the ladder for both columns (3uL ladder, 1 uL TBE buffer, and 1 uL of loading dye), and the rest of the wells contains sample 1-16 (4uL sample and 1uL of loading dye).
I ran a gel to see if all the reaction came out to be working. Even though the bands are not very bright for the left column (which is the first 8 samples), they are still working but just barely reached lateau. This might be caused by not well mixed tube setup. However, the gel looks fine, and the expriment can be continued.