Jie:LabNotes/CpgSeq/2009-7-27: Difference between revisions
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===PCR=== | ===PCR=== | ||
The primers | The primers: [[Media: 20090727_MMP9_ASE|MMP9_ASE_primer]] | ||
x 4 | x 4 | ||
2x iQ supermix 40 160 | 2x iQ supermix 40 160 | ||
Line 12: | Line 12: | ||
96C 3min-> 45x (95C 30sec, 58C or 62C 1min, 72C 1min)-> 72C 5min. | 96C 3min-> 45x (95C 30sec, 58C or 62C 1min, 72C 1min)-> 72C 5min. | ||
[[Image:20090727_PGP1F_PGP1L_MMP9_ASE.jpg]]20090727_PGP1F_PGP1L_MMP9_ASE | |||
The sanger sequencing results did not show ASE of MMP9 in two cell lines. |
Revision as of 18:33, 28 July 2009
MMP9 ASE of PGP1F and PGP1L
PCR
The primers: MMP9_ASE_primer x 4 2x iQ supermix 40 160 Primer F (100uM) 0.4 1.6 each Primer R (100uM) 0.4 1.6 each cDNA samples as above: 0.1 dH2O 40 160 total 80ul
96C 3min-> 45x (95C 30sec, 58C or 62C 1min, 72C 1min)-> 72C 5min.
File:20090727 PGP1F PGP1L MMP9 ASE.jpg20090727_PGP1F_PGP1L_MMP9_ASE
The sanger sequencing results did not show ASE of MMP9 in two cell lines.