Jie:LabNotes/CpgSeq/2009-7-27: Difference between revisions
Jump to navigation
Jump to search
>Jie deng No edit summary |
>Jie deng No edit summary |
||
Line 13: | Line 13: | ||
96C 3min-> 45x (95C 30sec, 58C or 62C 1min, 72C 1min)-> 72C 5min. | 96C 3min-> 45x (95C 30sec, 58C or 62C 1min, 72C 1min)-> 72C 5min. | ||
[[Image:20090727_PGP1F_PGP1L_MMP9_ASE.jpg| | [[Image:20090727_PGP1F_PGP1L_MMP9_ASE.jpg|300px]]20090727_PGP1F_PGP1L_MMP9_ASE | ||
The sanger sequencing results did not show ASE of MMP9 in two cell lines. | The sanger sequencing results did not show ASE of MMP9 in two cell lines. |
Revision as of 18:35, 28 July 2009
MMP9 ASE of PGP1F and PGP1L
PCR
The primers: MMP9_ASE_primer x 4 2x iQ supermix 40 160 Primer F (100uM) 0.4 1.6 each Primer R (100uM) 0.4 1.6 each cDNA samples as above: 0.1 dH2O 40 160 total 80ul
96C 3min-> 45x (95C 30sec, 58C or 62C 1min, 72C 1min)-> 72C 5min.
File:20090727 PGP1F PGP1L MMP9 ASE.jpg20090727_PGP1F_PGP1L_MMP9_ASE
The sanger sequencing results did not show ASE of MMP9 in two cell lines.