Kun:LabNotes/ExonomeSeq/2008-1-16: Difference between revisions

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Line 4: Line 4:
     10X Ampligase buffer                    1.5ul          1ul
     10X Ampligase buffer                    1.5ul          1ul


95C 10min -> 60C 40h -> add 2.5ul NSL mix (Stoffel 2U/ul, AmpLigase 0.5U/ul, dNTP 20uM) -> 60C 3h - -> 37C 1min -> add 1ul Exo I&&III, 1ul T7 exo, 37C 2h -> 94C 5min -> 4C hold.
95C 10min -> 60C 40h -> add 2.5ul NSL mix (Stoffel 2U/ul, AmpLigase 0.5U/ul, dNTP 50uM) -> 60C 1h - -> 37C 1min -> add 1ul Exo I&&III, 1ul T7 exo, 37C 2h -> 94C 5min -> 4C hold.

Latest revision as of 18:24, 18 January 2008

Exp.1 Perform another round of exon capture experiment[edit]

   NA10835(347ng/ul)                         3ul          3ul
   E55kv1 probes 180nM (01/12/08,purified)  10ul  275nM   7ul
   10X Ampligase buffer                    1.5ul          1ul

95C 10min -> 60C 40h -> add 2.5ul NSL mix (Stoffel 2U/ul, AmpLigase 0.5U/ul, dNTP 50uM) -> 60C 1h - -> 37C 1min -> add 1ul Exo I&&III, 1ul T7 exo, 37C 2h -> 94C 5min -> 4C hold.