Jie:LabNotes/CpgSeq/2009-8-7: Difference between revisions
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>Sam Chiang (New page: ==breast cancer patient peripheral blood DNA sample capture by cpg97k== ===Bisulfite conversion of patient DNA === {| border="1" cellpadding="5" cellspacing="0" align="center" |- | align=...) |
>Sam Chiang No edit summary |
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==breast cancer patient peripheral blood DNA sample capture by cpg97k== | ==breast cancer patient peripheral blood DNA sample capture by cpg97k== | ||
===gDNA extraction from blood=== | |||
I used the Qiagen FlexiGene DNA kit to exact the DNA from blood. yield: | |||
05192A18: 83.5ng/ul x 100ul; | |||
05192B09: 72.7ng/ul x 100ul | |||
===Bisulfite conversion of patient DNA === | ===Bisulfite conversion of patient DNA === | ||
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| align="center" style="background:#f0f0f0;"|'''conversed DNA concentration and volumn''' | | align="center" style="background:#f0f0f0;"|'''conversed DNA concentration and volumn''' | ||
|- | |- | ||
||| | |||05192A18||83.5ng/ul x 1 tube||20ul||0ul||130ul||148.2ng/ul x 10ul | ||
|- | |- | ||
||| | |||05192B09||72.7ng/ulx 1 tubes||20ul||0ul||130ul||127.8ng/ul x 10ul | ||
|- | |- | ||
|} | |} |
Revision as of 01:29, 8 August 2009
breast cancer patient peripheral blood DNA sample capture by cpg97k
gDNA extraction from blood
I used the Qiagen FlexiGene DNA kit to exact the DNA from blood. yield: 05192A18: 83.5ng/ul x 100ul; 05192B09: 72.7ng/ul x 100ul
Bisulfite conversion of patient DNA
No | sample | sample concentration | sample volumn | ddH2O | conversion reagents | conversed DNA concentration and volumn |
05192A18 | 83.5ng/ul x 1 tube | 20ul | 0ul | 130ul | 148.2ng/ul x 10ul | |
05192B09 | 72.7ng/ulx 1 tubes | 20ul | 0ul | 130ul | 127.8ng/ul x 10ul |