Jie:LabNotes/CpgSeq/2009-8-7: Difference between revisions

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>Sam Chiang
(New page: ==breast cancer patient peripheral blood DNA sample capture by cpg97k== ===Bisulfite conversion of patient DNA === {| border="1" cellpadding="5" cellspacing="0" align="center" |- | align=...)
 
>Sam Chiang
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==breast cancer patient peripheral blood DNA sample capture by cpg97k==
==breast cancer patient peripheral blood DNA sample capture by cpg97k==
===gDNA extraction from blood===
I used the Qiagen FlexiGene DNA kit to exact the DNA from blood. yield:
05192A18: 83.5ng/ul x 100ul;
05192B09: 72.7ng/ul x 100ul
===Bisulfite conversion of patient DNA ===
===Bisulfite conversion of patient DNA ===


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| align="center" style="background:#f0f0f0;"|'''conversed DNA concentration and volumn'''
| align="center" style="background:#f0f0f0;"|'''conversed DNA concentration and volumn'''
|-
|-
|||5028||320ng/ul x 1 tube||20ul||0ul||130ul||148.2ng/ul x 10ul
|||05192A18||83.5ng/ul x 1 tube||20ul||0ul||130ul||148.2ng/ul x 10ul
|-
|-
|||4735||260ng/ulx 1 tubes||20ul||0ul||130ul||127.8ng/ul x 10ul
|||05192B09||72.7ng/ulx 1 tubes||20ul||0ul||130ul||127.8ng/ul x 10ul
|-
|-
|}
|}

Revision as of 01:29, 8 August 2009

breast cancer patient peripheral blood DNA sample capture by cpg97k

gDNA extraction from blood

I used the Qiagen FlexiGene DNA kit to exact the DNA from blood. yield:
05192A18: 83.5ng/ul x 100ul;
05192B09: 72.7ng/ul x 100ul

Bisulfite conversion of patient DNA

No sample sample concentration sample volumn ddH2O conversion reagents conversed DNA concentration and volumn
05192A18 83.5ng/ul x 1 tube 20ul 0ul 130ul 148.2ng/ul x 10ul
05192B09 72.7ng/ulx 1 tubes 20ul 0ul 130ul 127.8ng/ul x 10ul