AlanFung:LabNotes/DNA/2009-8-18: Difference between revisions
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Line 12: | Line 12: | ||
| 10X NEBuffer 4||4||4||4||3||3 | | 10X NEBuffer 4||4||4||4||3||3 | ||
|- | |- | ||
| 1mM SAM (fresh)|| | | 1mM SAM (fresh)||8||8||8||6||6 | ||
|- | |- | ||
| 2U/ul MmeI||1||1||1||1||1 | | 2U/ul MmeI||1||1||1||1||1 | ||
|- | |- | ||
| ddH20|| | | ddH20||7||7||7||10||10 | ||
|- | |- | ||
| Total||40||40||40||30||30 | | Total||40||40||40||30||30 |
Revision as of 19:26, 18 August 2009
Digestion with MmeI
PGP4 | PGP6 | PGP10 | A | B | |
Sample | 20 | 20 | 20 | 10 | 10 |
10X NEBuffer 4 | 4 | 4 | 4 | 3 | 3 |
1mM SAM (fresh) | 8 | 8 | 8 | 6 | 6 |
2U/ul MmeI | 1 | 1 | 1 | 1 | 1 |
ddH20 | 7 | 7 | 7 | 10 | 10 |
Total | 40 | 40 | 40 | 30 | 30 |
1mM SAM: 32mM SAM 1ul + 31ul ddH2O. 37C 2h.
- Qiaquick column purification. Elute in 12ul EB.