AlanFung:LabNotes/DNA/2009-8-18: Difference between revisions

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>Alan6017518
Line 12: Line 12:
| 10X NEBuffer 4||4||4||4||3||3
| 10X NEBuffer 4||4||4||4||3||3
|-
|-
| 1mM SAM (fresh)||4||4||4||3||3
| 1mM SAM (fresh)||8||8||8||6||6
|-
|-
| 2U/ul MmeI||1||1||1||1||1
| 2U/ul MmeI||1||1||1||1||1
|-
|-
| ddH20||11||11||11||13||13
| ddH20||7||7||7||10||10
|-
|-
| Total||40||40||40||30||30
| Total||40||40||40||30||30

Revision as of 19:26, 18 August 2009

Digestion with MmeI

PGP4 PGP6 PGP10 A B
Sample 20 20 20 10 10
10X NEBuffer 4 4 4 4 3 3
1mM SAM (fresh) 8 8 8 6 6
2U/ul MmeI 1 1 1 1 1
ddH20 7 7 7 10 10
Total 40 40 40 30 30
 1mM SAM: 32mM SAM 1ul + 31ul ddH2O.
 37C 2h. 
  • Qiaquick column purification. Elute in 12ul EB.