Jie:LabNotes/CpgSeq/2009-8-26: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Jie deng
(New page: ==ASM validation of cell type and individual dependent ASM== primers for cell and individual dependent ASM validation I did the PCR...)
 
>Jie deng
No edit summary
Line 4: Line 4:
  I did the PCR using 2x iQ supermix on:
  I did the PCR using 2x iQ supermix on:
   
   
  rs10846023_FLJ22662 on BJ and BJiPS11
  rs10846023_FLJ22662 on BJ and BJiPS11;
MATN4 on PGP1F and PGP1iPS;
DNMT3B on PGP3L and PGP9L;
                                                          x 7
2x iQ supermix                          50            350         
Primer F (50uM) 0.5         0.5 each
Primer R (50uM) 0.5            0.5 each
sample bis-gDNA (~202ng/ul)            0.1   0.1 each
dH2O         50 350
total         40ul
 
96C 3min-> 40x (95C 30sec, 62C 1min, 72C 1min)-> 72C 5min.

Revision as of 23:29, 26 August 2009

ASM validation of cell type and individual dependent ASM

primers for cell and individual dependent ASM validation 
I did the PCR using 2x iQ supermix on:

rs10846023_FLJ22662 on BJ and BJiPS11;
MATN4 on PGP1F and PGP1iPS;
DNMT3B on PGP3L and PGP9L; 
                                                         x 7
2x iQ supermix                          50             350          
Primer F (50uM)			 0.5	        0.5 each
Primer R (50uM)			 0.5            0.5 each
sample bis-gDNA (~202ng/ul)             0.1	  	0.1 each
dH2O				         50		350
total				         40ul
96C 3min-> 40x (95C 30sec, 62C 1min, 72C 1min)-> 72C 5min.