Jie:LabNotes/CpgSeq/2009-8-26: Difference between revisions

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  I did the PCR using 2x iQ supermix on:
  I did the PCR using 2x iQ supermix on:
   
   
  rs10846023_FLJ22662 on BJ and BJiPS11;
  rs10846023_FLJ22662 on BJ(7/26/09) and BJiPS11(;
  MATN4 on PGP1F and PGP1iPS;
  MATN4 on PGP1F(10/10/08) and PGP1iPS;
  DNMT3B on PGP3L and PGP9L;  
  DNMT3B on PGP3L and PGP9L;  
                                                           x 7
                                                           x 7
Line 15: Line 15:
  total         40ul
  total         40ul


  96C 3min-> 40x (95C 30sec, 62C 1min, 72C 1min)-> 72C 5min.
  96C 3min-> 45x (95C 30sec, MATN4 and DNMT3B at 58C, rs10846023 on 62C 1min, 72C 1min)-> 72C 5min.

Revision as of 00:29, 27 August 2009

ASM validation of cell type and individual dependent ASM

primers for cell and individual dependent ASM validation 
I did the PCR using 2x iQ supermix on:

rs10846023_FLJ22662 on BJ(7/26/09) and BJiPS11(;
MATN4 on PGP1F(10/10/08) and PGP1iPS;
DNMT3B on PGP3L and PGP9L; 
                                                         x 7
2x iQ supermix                          50             350          
Primer F (50uM)			 0.5	        0.5 each
Primer R (50uM)			 0.5            0.5 each
sample bis-gDNA (~202ng/ul)             0.1	  	0.1 each
dH2O				         50		350
total				         40ul
96C 3min-> 45x (95C 30sec, MATN4 and DNMT3B at 58C, rs10846023 on 62C 1min, 72C 1min)-> 72C 5min.