Jie:LabNotes/CpgSeq/2009-8-27: Difference between revisions

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>Jie deng
(New page: ==ASM validation of cell type and individual dependent ASM== primers for cell and individual dependent ASM validation I did the PCR...)
 
>Jie deng
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  chr12:4541690 on PGP1L and PGP1F;
  chr12:4541690 on PGP1L and PGP1F;
  chr21:32746340 on PGP1L and PGP1F;
  chr21:32746340 on PGP1L and PGP1F;
  chr20:60061824 on PGP3L and PGP9L;
  chr20:60061824 on PGP3L and PGP9L;
  chr20:60403281 on PGP1L and PGP3L;  
  chr20:60403281 on PGP1L and PGP3L;  
                                                           x 8
                                                           x 8
  2x iQ supermix                          50            400           
  2x iQ supermix                          50            400           

Revision as of 20:17, 27 August 2009

ASM validation of cell type and individual dependent ASM

primers for cell and individual dependent ASM validation 
I did the PCR using 2x iQ supermix on:

chr12:4541690 on PGP1L and PGP1F;
chr21:32746340 on PGP1L and PGP1F;
chr20:60061824 on PGP3L and PGP9L;
chr20:60403281 on PGP1L and PGP3L; 
                                                         x 8
2x iQ supermix                          50             400          
Primer F (50uM)			 0.5	        0.5 each
Primer R (50uM)			 0.5            0.5 each
sample bis-gDNA (~202ng/ul)             0.1	  	0.1 each
dH2O				         50		400
total				         40ul
96C 3min-> 45x (95C 30sec, MATN4 and DNMT3B at 58C, rs10846023 on 62C 1min, 72C 1min)-> 72C 5min.