Jie:LabNotes/CpgSeq/2009-8-27: Difference between revisions
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>Jie deng (New page: ==ASM validation of cell type and individual dependent ASM== primers for cell and individual dependent ASM validation I did the PCR...) |
>Jie deng No edit summary |
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chr12:4541690 on PGP1L and PGP1F; | chr12:4541690 on PGP1L and PGP1F; | ||
chr21:32746340 on PGP1L and PGP1F; | chr21:32746340 on PGP1L and PGP1F; | ||
chr20:60061824 on PGP3L and PGP9L; | chr20:60061824 on PGP3L and PGP9L; | ||
chr20:60403281 on PGP1L and PGP3L; | chr20:60403281 on PGP1L and PGP3L; | ||
x 8 | x 8 | ||
2x iQ supermix 50 400 | 2x iQ supermix 50 400 |
Revision as of 20:17, 27 August 2009
ASM validation of cell type and individual dependent ASM
primers for cell and individual dependent ASM validation
I did the PCR using 2x iQ supermix on: chr12:4541690 on PGP1L and PGP1F; chr21:32746340 on PGP1L and PGP1F; chr20:60061824 on PGP3L and PGP9L; chr20:60403281 on PGP1L and PGP3L;
x 8 2x iQ supermix 50 400 Primer F (50uM) 0.5 0.5 each Primer R (50uM) 0.5 0.5 each sample bis-gDNA (~202ng/ul) 0.1 0.1 each dH2O 50 400 total 40ul
96C 3min-> 45x (95C 30sec, MATN4 and DNMT3B at 58C, rs10846023 on 62C 1min, 72C 1min)-> 72C 5min.