Jie:LabNotes/CpgSeq/2009-9-2: Difference between revisions

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>Sam Chiang
(New page: ==ASM validation of imprinted genes== HUES42: 81.1ng/ul HUES63: 36.5ng/ul; hFib2: 16.7ng/ul)
 
>Jie deng
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==ASM validation of imprinted genes==
==ASM validation of imprinted genes==
HUES42: 81.1ng/ul
===I did the bisulfite conversion of HUES42, HUES63 and hFib2===
HUES63: 36.5ng/ul;
  The concentration after bisulfite conversion using zymo kit:
hFib2: 16.7ng/ul
  HUES42: 81.1ng/ul
  HUES63: 36.5ng/ul;
  hFib2: 16.7ng/ul
 
===PCR with SLC26A10 and SNRPN primers===
SLC26A10: HUES42 and hFib2;
SNRPN: HUES63;
 
===TA cloning of the PCR products from the above three samples===
I send the plate SLC26A10_hFib2_HUES42_SNRPN_Hues63.
A1-C8:SLC26A10_hFib2; C9-F4:SLC26A10_HUES42; F5-H12:SNRPN_Hues63.

Latest revision as of 17:06, 25 September 2009

ASM validation of imprinted genes[edit]

I did the bisulfite conversion of HUES42, HUES63 and hFib2[edit]

 The concentration after bisulfite conversion using zymo kit:
 HUES42: 81.1ng/ul
 HUES63: 36.5ng/ul;
 hFib2: 16.7ng/ul

PCR with SLC26A10 and SNRPN primers[edit]

SLC26A10: HUES42 and hFib2;
SNRPN: HUES63;

TA cloning of the PCR products from the above three samples[edit]

I send the plate SLC26A10_hFib2_HUES42_SNRPN_Hues63.
A1-C8:SLC26A10_hFib2; C9-F4:SLC26A10_HUES42; F5-H12:SNRPN_Hues63.