Athurva Gore/LabNotes/2009-9-9: Difference between revisions
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=IPS and Cancer= | =IPS and Cancer= | ||
==LOH Mutations== | |||
* Need to check LOH mutations; is the new variant the damaging one? Or the old one? | * Need to check LOH mutations; is the new variant the damaging one? Or the old one? | ||
** If nondamaging version is lost, this is not actually harmful to the cell... | ** If nondamaging version is lost, this is not actually harmful to the cell... | ||
** Modified SIFT input to have fibroblast first, IPS second | ** Modified SIFT input to have fibroblast first, IPS second | ||
** Can now easily check if damage is gained or lost. | ** Can now easily check if damage is gained or lost. | ||
* ALL DAMAGING MUTATIONS: The non-damaging variant was lost via LOH! | |||
** Strange. Will need to investigate with Primers... | |||
==PCR Primers for target Genes== | |||
* Need to use Primer3 to obtain PCR primers for regions of interest. | |||
** Have five regions of interest: 2 from GOH, 3 from LOH |
Revision as of 21:13, 9 September 2009
Exome Resequencing
IPS and Cancer
LOH Mutations
- Need to check LOH mutations; is the new variant the damaging one? Or the old one?
- If nondamaging version is lost, this is not actually harmful to the cell...
- Modified SIFT input to have fibroblast first, IPS second
- Can now easily check if damage is gained or lost.
- ALL DAMAGING MUTATIONS: The non-damaging variant was lost via LOH!
- Strange. Will need to investigate with Primers...
PCR Primers for target Genes
- Need to use Primer3 to obtain PCR primers for regions of interest.
- Have five regions of interest: 2 from GOH, 3 from LOH